Article
Spiribacter curvatus sp. nov, a moderately halophilic bacterium isolated from a saltern
Author/s | León León, María José
Rodríguez-Olmos, Ángel Sánchez-Porro Álvarez, Cristina López Pérez, Mario Rodríguez Valera, Francisco Soliveri, Juan Ventosa Ucero, Antonio Copa-Patiño, José Luis |
Department | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Publication Date | 2015-12 |
Deposit Date | 2024-09-24 |
Published in |
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Abstract | A novel pink-pigmented bacterial strain, UAH-SP71T, was isolated from a saltern located in Santa Pola, Alicante (Spain) and the complete genome sequence was analysed and compared with that of Spiribacter salinus M19-40T, ... A novel pink-pigmented bacterial strain, UAH-SP71T, was isolated from a saltern located in Santa Pola, Alicante (Spain) and the complete genome sequence was analysed and compared with that of Spiribacter salinus M19-40T, suggesting that the two strains constituted two separate species, with a 77.3% ANI value. In this paper, strain UAH-SP71T was investigated in a taxonomic study using a polyphasic approach. Strain UAH-SP71T was a Gram-stain-negative, strictly aerobic, non-motile curved rod that grew in media containing 5–20% (w/v) NaCl (optimum 10% NaCl), at 5–40 °C (optimum 37 8C) and at pH 5–10 (optimum pH 8). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain UAH-SP71T is a member of the genus Spiribacter, showing a sequence similarity of 96.5% with Spiribacter salinus M19-40T. Other related species are also members of the family Ectothiorhodospiraceae, including Arhodomonas recens RS91T (95.5% 16S rRNA gene sequence similarity), Arhodomonas aquaeolei ATCC 49307T (95.4 %) and Alkalilimnicola ehrlichii MLHE-1T (94.9 %). DNA–DNA hybridization between strain UAH-SP71T and Spiribacter salinus M19-40T was 39 %. The major cellular fatty acids of strain UAH-SP71T were C18 : 1ω6c and/or C18 : 1ω7c, C16 : 0, C16 : 1ω6c and/or C16 : 1ω7c, C10 : 0 3-OH and C12 : 0, a pattern similar to that of Spiribacter salinus M19-40T. Phylogenetic, phenotypic and genotypic differences between strain UAH-SP71T and Spiribacter salinus M19-40T indicate that strain UAH-SP71T represents a novel species of the genus Spiribacter, for which the name Spiribacter curvatus sp. Nov. is proposed. The type strain is UAH-SP71T (=CECT 8396T =DSM 28542T). |
Funding agencies | Ministerio de Economía y Competitividad (MINECO). España Junta de Andalucía |
Project ID. | CGL2013-46941-P
P10-CVI-6226 |
Citation | León León, M.J., Rodríguez-Olmos, Á., Sánchez-Porro Álvarez, C., López Pérez, M., Rodríguez Valera, F., Soliveri, J.,...,Copa-Patiño, J.L. (2015). Spiribacter curvatus sp. nov, a moderately halophilic bacterium isolated from a saltern. International Journal of Systematic and Evolutionary Microbiology, 65 (12), 4638-4643. https://doi.org/10.1099/ijsem.0.000621. |
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