Article
Marine Plant Growth Promoting Bacteria (PGPB) Inoculation Technology: Testing the Effectiveness of Different Application Methods to Improve Tomato Plants Tolerance Against Acute Heat Wave Stress
Author/s | Duarte, Bernardo
Carreiras, João Albuquerque Cruz Silva, Ana Mateos Naranjo, Enrique Rodríguez Llorente, Ignacio David Pajuelo Domínguez, Eloísa Redondo Gómez, Susana Mesa Marín, Jennifer Figueiredo, Andreia |
Department | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Publication Date | 2024 |
Deposit Date | 2024-06-11 |
Published in |
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Abstract | Regenerative agriculture aims to boost native biodiversity and ensure sustainable production. Plant Growth Promoting Bacteria (PGPB) enhance crop yield through improved nutrient use efficiency or stress tolerance. Utilizing ... Regenerative agriculture aims to boost native biodiversity and ensure sustainable production. Plant Growth Promoting Bacteria (PGPB) enhance crop yield through improved nutrient use efficiency or stress tolerance. Utilizing synthetic bacterial communities (SynCom) tailored to specific challenges holds promise for enhancing plant resilience. However, challenges include designing microbial consortia and selecting SynCom application techniques. This study tested four PGPB SynCom application methods on tomato plants to evaluate their effectiveness in promoting plant resistance and recovery from short extreme heat waves. Non-invasive phenotyping techniques were used to assess plant responses to three delivery methods (watering, foliar spray, and alginate spheres immobilization) under heat stress. Plants treated with the marine PGPB SynCom through methods like leaf spraying or encapsulation in alginate spheres exhibited improved resilience in terms of Kautsky curve intensity and shape, indicating better photochemical apparatus function. These techniques also facilitated post-stress recovery. Deep photochemical analysis revealed SynCom-induced improvements in the plant's photochemical apparatus, particularly the PS II donor side. Moreover, it was also possible to observe that upon stress relief these two techniques, alongside the plants inoculated throughout watering were able to recover their photochemical profiles. Analysing the Fv/Fm values, plants inoculated through watering, alginate beads and leaf spray can be considered heat-tolerant, a characteristic conserved throughout the three sampling moments. Heat-wave exposure led to an increase in the energy absorbed by the non-inoculated plants as a counteractive measure to overcome the low energy use efficiency observed (low trapping and transported energy fluxes). The reduction in stress impact was attributed to SynCom's ACC-deaminase production, which lowered ethylene accumulation, promoting growth and photosynthetic efficiency. The study also noted reduced energy dissipation under stress relief, indicating efficient heat dissipation mechanisms due to SynCom application. Statistical models generated with the attained photochemical data allowed depicting specific fluorescence signatures of each thermal and inoculation treatment with a very high degree of accuracy, reinforcing that these different inoculation treatments have in fact different impacts on plant photochemistry but also provide a tool for future phenotyping assessments using the proposed inoculation techniques. Alginate bead based PGPB release emerged as an efficient, scalable technique with continuous benefits for plant growth and stress response. |
Funding agencies | Fundação para a Ciência e a Tecnologia. Portugal Junta de Andalucía |
Project ID. | 20114–2
PRIMA/0011/2020 2022.01746.CEECIND 2022.11260.BD UI/BD/153050/2022 DOC_00725 |
Citation | Duarte, B., Carreiras, J.A., Cruz Silva, A., Mateos Naranjo, E., Rodríguez Llorente, I.D., Pajuelo Domínguez, E.,...,Figueiredo, A. (2024). Marine Plant Growth Promoting Bacteria (PGPB) Inoculation Technology: Testing the Effectiveness of Different Application Methods to Improve Tomato Plants Tolerance Against Acute Heat Wave Stress. Plant Stress, 11, 100434. https://doi.org/10.1016/j.stress.2024.100434. |
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