Article
Glasses-Assisted 3D Display System–Guided Descemet Membrane Endothelial Keratoplasty Tissue Preparation
Author/s | Borroni, Davide
Rocha de Lossada, Carlos Bonci, Paola Rechichi, Miguel Rodríguez Calvo de Mora, Marina Rachwani-Anil, Rahul Sánchez González, José María Urbinati, Facundo García Lorente, María Carones, Francesco |
Department | Universidad de Sevilla. Departamento de Física de la Materia Condensada |
Publication Date | 2022 |
Deposit Date | 2024-06-10 |
Published in |
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Abstract | Purpose: The aim of this study was to evaluate the feasibility of Descemet membrane endothelial keratoplasty (DMEK) tissue prep- aration using a glasses-assisted 3-dimensional (3D) display system and to compare it with a ... Purpose: The aim of this study was to evaluate the feasibility of Descemet membrane endothelial keratoplasty (DMEK) tissue prep- aration using a glasses-assisted 3-dimensional (3D) display system and to compare it with a conventional surgical microscope. Methods: Healthy pairs of human corneas suitable for penetrating keratoplasty surgery were selected for this study. The tissues were randomly divided into 2 groups. Each pair of corneas had 1 cornea (group 1) prepared with NGENUITY (Alcon) with a 5-second staining time with vision blue, and the fellow cornea (group 2) was prepared using a OPMI Lumera 700 surgical microscope (Carl Zeiss Meditec, Jena, Germany) with a 30-second staining time. DMEK graft preparation time, speed of stripping, graft width, and endothe- lial cell loss were evaluated. Results: Twenty-eight pairs of corneas were included in this study. The graft preparation time was significantly higher in the 3D group than in the conventional group (498 6 147 vs. 418 6 85 seconds, P value = 0.031). The mean speed of stripping was 0.59 6 0.081 mm/s in group 1 and 0.089 6 0.005 mm/s in group 2 (P value = 0.024). The mean endothelial cell density in group 1 and group 2 before tissue preparation was 2162 6 115.21 and 2153 6 122.45, respectively (P value . 0.1). After tissue preparation, the endothelial cell density reduced to 1911 6 150.72 in group 1 and 1998 6 90.72 in group 2 (P value = P value . 0.05). The graft width was 5.05 6 0.71 mm in group 1 and 4.92 6 0.23 mm in group 2 (P value . 0.05). Conclusions: DMEK tissue preparation with 3D display system NGENUITY is feasible with a slightly increased preparation time. The improved visualization allows a reduced staining time that could |
Citation | Borroni, D., Rocha de Lossada, C., Bonci, P., Rechichi, M., Rodríguez Calvo de Mora, M., Rachwani-Anil, R.,...,Carones, F. (2022). Glasses-Assisted 3D Display System–Guided Descemet Membrane Endothelial Keratoplasty Tissue Preparation. Cornea, 41 (11), 1444-1446. https://doi.org/10.1097/ICO.0000000000003005. |
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