Artículo
Three Genes Involved in Different Signaling Pathways, carS, wcoA, and acyA, Participate in the Regulation of Fusarin Biosynthesis in Fusarium fujikuroi
Autor/es | Díaz Sánchez, Violeta
Castrillo, Marta García Martínez, Jorge Avalos, Javier Limón Mirón, María del Carmen |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2024 |
Fecha de depósito | 2024-05-31 |
Publicado en |
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Resumen | The phytopathogenic fungus Fusarium fujikuroi has a rich secondary metabolism which includes the synthesis of very different metabolites in response to diverse environmental cues, such as light or nitrogen. Here, we focused ... The phytopathogenic fungus Fusarium fujikuroi has a rich secondary metabolism which includes the synthesis of very different metabolites in response to diverse environmental cues, such as light or nitrogen. Here, we focused our attention on fusarins, a class of mycotoxins whose synthesis is downregulated by nitrogen starvation. Previous data showed that mutants of genes involved in carotenoid regulation (carS, encoding a RING finger protein repressor), light detection (wcoA, White Collar photoreceptor), and cAMP signaling (AcyA, adenylate cyclase) affect the synthesis of different metabolites. We studied the effect of these mutations on fusarin production and the expression of the fus1 gene, which encodes the key polyketide synthase of the pathway. We found that the three proteins are positive regulators of fusarin synthesis, especially WcoA and AcyA, linking light regulation to cAMP signaling. Genes for two other photoreceptors, the cryptochrome CryD and the Vivid flavoprotein VvdA, were not involved in fusarin regulation. In most cases, there was a correspondence between fusarin production and fus1 mRNA, indicating that regulation is mainly exerted at the transcriptional level. We conclude that fusarin synthesis is subject to a complex control involving regulators from different signaling pathways. |
Agencias financiadoras | Plan Propio de la Universidad de Sevilla Ministerio de Ciencia e Innovación (MICIN). España Agencia Estatal de Investigación. España Plan Andaluz de Investigación, Desarrollo e Innovación (Junta de Andalucía) European Regional Development Fund (ERDF) |
Identificador del proyecto | BIO2009-11131
PID2022-140414NB-I00 P07-CVI-02813 RED2022-134577-T |
Cita | Díaz Sánchez, V., Castrillo, M., García Martínez, J., Avalos, J. y Limón Mirón, M.d.C. (2024). Three Genes Involved in Different Signaling Pathways, carS, wcoA, and acyA, Participate in the Regulation of Fusarin Biosynthesis in Fusarium fujikuroi. Journal of Fungi, 10 (3), 203. https://doi.org/10.3390/jof10030203. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Three Genes_Carmen Limón.pdf | 2.232Mb | [PDF] | Ver/ | |