Artículo
Effects of polyphenolic maqui ( Aristotelia chilensis ) extract on the inhibition of NLRP3 inflammasome and activation of mast cells in a mouse model of Crohn's disease-like colitis
Autor/es | Ortiz Cerda, Tamara Andrea
Argüelles Arias, Federico Macías García, Laura Vázquez Román, María Victoria Tapia, Gladys Xie, Kangzhe García-García, María Desirée Merinero de los Santos, Manuel García Montes, Josefa María Alcudia Cruz, Ana Witting, Paul K. Miguel Rodríguez, Manuel de |
Departamento | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2024-01-12 |
Fecha de depósito | 2024-05-29 |
Publicado en |
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Resumen | Introduction: Crohn’s disease (CD) involves activation of mast cells (MC) and NFкB in parallel with the PPAR-a/NLRP3 inflammasome/IL-1b pathway in the
inflamed colon. Whether polyphenols from maqui (Aristotelia ... Introduction: Crohn’s disease (CD) involves activation of mast cells (MC) and NFкB in parallel with the PPAR-a/NLRP3 inflammasome/IL-1b pathway in the inflamed colon. Whether polyphenols from maqui (Aristotelia chilensis) represent a natural alternative treatment for CD is unclear. Therefore, we used an animal model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD-like colitis to investigate protective effects of maqui extract through monitoring NLRP3 inflammasome and MC activation in colon tissue. Methods: Maqui extract was administered via orogastric route to mice after (post-Treatment group) or prior (pre-Treatment group) to TNBS-induction. Colon pathology was characterized by histoarchitectural imaging, disease activity index (DAI), and assessing NF-кB, p-NF-кB, PPAR-a/NLRP3 expression and IL-1b levels. Results: Compared to mice treated with TNBS alone administration of anthocyanin-rich maqui extract improved the DAI, colon histoarchitecture and reduced both colon wet-weight and transmural inflammation. Induction with TNBS significantly increased colonic NLPR3 inflammasome activation, while cotreatment with maqui extract (either post- or pre-Treatment) significantly downregulated NLRP3, ASC and caspase-1 levels, which manifested as reduced colonic IL-1b levels. Supplemented maqui extract marginally diminished NF-кB activity in epithelial cells but reached statistical significance in immune cells (as judged by decreased NF-кB phosphorylation). PPAR-a signaling was largely unaffected by Maqui whereas MC infiltration into the colon mucosa and submucosa decreased and their level of degranulation was suppressed. Conclusion: These outcomes show the post- and pre- Treatment effect of a polyphenolic extract rich in anthocyanins from maqui the acute phase of TNBSinduced CD-like colitis is linked to suppression of the NLRP3 inflammasome and reduced MC responses. These data indicate that maqui extract represents a potential nutraceutical for the treatment of inflammatory bowel disease (IBD). |
Agencias financiadoras | Junta de Andalucía |
Identificador del proyecto | PAIDI CTS949 |
Cita | Ortiz Cerda, T.A., Argüelles Arias, F., Macías García, L., Vázquez Román, M.V., Tapia, G., Xie, K.,...,Miguel Rodríguez, M.d. (2024). Effects of polyphenolic maqui ( Aristotelia chilensis ) extract on the inhibition of NLRP3 inflammasome and activation of mast cells in a mouse model of Crohn's disease-like colitis. Frontiers In Immunology, 14, 1229767. https://doi.org/10.3389/fimmu.2023.1229767. |
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