Artículo
Effect of different crosslinking agents on hybrid chitosan/collagen hydrogels for potential tissue engineering applications
Autor/es | Sánchez Cid, Pablo
Alonso González, María Jiménez-Rosado, Mercedes Rafii-El-Idrissi Benhnia, Mohammed Ruiz-Mateos Carmona, Ezequiel Ostos Marcos, Francisco José Romero García, Alberto Pérez-Puyana, Víctor Manuel |
Departamento | Universidad de Sevilla. Departamento de Ingeniería Química Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología |
Fecha de publicación | 2024-04 |
Fecha de depósito | 2024-05-28 |
Publicado en |
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Resumen | Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting ... Tissue engineering (TE) demands scaffolds that have the necessary resistance to withstand the mechanical stresses once implanted in our body, as well as excellent biocompatibility. Hydrogels are postulated as interesting materials for this purpose, especially those made from biopolymers. In this study, the microstructure and rheological performance, as well as functional and biological properties of chitosan and collagen hydrogels (CH/CG) crosslinked with different coupling agents, both natural such as d-Fructose (F), genipin (G) and transglutaminase (T) and synthetic, using a combination of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with N-hydroxysuccinimide (EDC/NHS) will be assessed. FTIR tests were carried out to determine if the proposed crosslinking reactions for each crosslinking agent occurred as expected, obtaining positive results in this aspect. Regarding the characterization of the properties of each system, two main trends were observed, from which it could be established that crosslinking with G and EDC-NHS turned out to be more effective and beneficial than with the other two crosslinking agents, producing significant improvements with respect to the base CH/CG hydrogel. In addition, in vitro tests demonstrated the potential application in TE of these systems, especially for those crosslinked with G, T and EDC-NHS. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Junta de Andalucía Consejo Superior de Investigaciones Científicas (CSIC) |
Identificador del proyecto | PID2021-124294OB-C21
PAIDI DOCTOR 2019–2020 DOC_00586 PAIDI DOCTOR 2019–2020 DOC_00963 |
Cita | Sánchez Cid, P., Alonso González, M., Jiménez-Rosado, M., Rafii-El-Idrissi Benhnia, M., Ruiz-Mateos Carmona, E., Ostos Marcos, F.J.,...,Pérez-Puyana, V.M. (2024). Effect of different crosslinking agents on hybrid chitosan/collagen hydrogels for potential tissue engineering applications. International Journal of Biological Macromolecules, 263, part 2 (129858). https://doi.org/10.1016/j.ijbiomac.2024.129858. |
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IJBM_romero-garcia_2024_effect.pdf | 4.420Mb | [PDF] | Ver/ | |