Artículo
Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models
Autor/es | Gallego-Durán, Rocío
Álvarez-Amor, Leticia Gil-Gómez, Antonio Rojas, Ángela Muñoz Hernández, Rocío Cádernas-García, Antonio Ampuero Herrojo, Javier Romero Gómez, Manuel |
Departamento | Universidad de Sevilla. Departamento de Fisiología Universidad de Sevilla. Departamento de Medicina Instituto de Biomedicina de Sevilla (IBIS) |
Fecha de publicación | 2019 |
Fecha de depósito | 2024-05-15 |
Publicado en |
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Resumen | Introduction: non-alcoholic fatty liver disease is one of
the most prevalent liver disorders in the developed world.
Currently, there is no approved pharmacological therapy
except for lifestyle intervention. Therefore, ... Introduction: non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better thera peutic management. Material and methods: this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supple mented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was sub sequently evaluated. Results: HF-HFD animals showed an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had high er ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. Conclusion: in conclusion, the comparison of these mod els helped us to decide if it is better to select a long-term but more physiological model for physiopathology studies or either a more rapid NASH model for novel molecules testing. |
Cita | Gallego-Durán, R., Álvarez-Amor, L., Gil-Gómez, A., Rojas, Á., Muñoz Hernández, R., Cádernas-García, A.,...,Romero Gómez, M. (2019). Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models. Revista Española de Enfermedades Digestivas, 111 (4), 301-307. https://doi.org/10.17235/reed.2019.6083/2018. |
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