Trabajo Fin de Máster
CENP-V expression in human eggs and CENP-V depletion in Caenorhabditis elegans.
Autor/es | Vargas Fernández, Carmen María |
Director | Chacón Rodríguez, Mariola |
Departamento | Universidad de Sevilla. Departamento de Biología Celular. |
Fecha de publicación | 2023-07-05 |
Fecha de depósito | 2024-04-30 |
Titulación | Universidad de Sevilla. Máster Universitario en Biología Avanzada: Investigación y Aplicación. |
Resumen | CENP- V is a kinetochore protein needed for meiosis progression in mice oocytes.
Cenp-V -/- mice oocytes arrest at metaphase I or proceed to metaphase II harbouring lagging
chromosomes. The goal of this study is in one ... CENP- V is a kinetochore protein needed for meiosis progression in mice oocytes. Cenp-V -/- mice oocytes arrest at metaphase I or proceed to metaphase II harbouring lagging chromosomes. The goal of this study is in one hand to study CENP-V directly into the human egg and in parallel to study CENP-V in a model system in which oocyte availability is unlimited and meiotic prophase can be analysed. As a first step, we optimised the immunofluorescence of CENP-V in human oocytes from discard material of in vitro fertilisation treatment. We found that the pellucid zone needs to be removed to allow the penetrance of the antibody. Next, we have established a loss-of-function assay by RNAi in an alternative model, Caenorhabditis elegans. We find that the depletion of F25B4.8, the ortholog of CENP-V in the nematode causes chromosomes aberrations. Hence, we conclude that C.elegans can be used as a model to study the role of CENP-V during oocyte meiosis and thus to complement the analysis in the human egg. Moreover, the ortholog of Cenp-V has been never studied in the nematode before. |
Cita | Vargas Fernández, C.M. (2023). CENP-V expression in human eggs and CENP-V depletion in Caenorhabditis elegans.. (Trabajo Fin de Máster Inédito). Universidad de Sevilla, Sevilla. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Carmen María Vargas Fernández.pdf | 3.591Mb | [PDF] | Ver/ | |
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