Article
Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin
Author/s | Anjani, Qonita Kurnia
Pandya, Anjali K. Demartis, Sara Domínguez Robles, Juan Moreno Castellanos, Natalia Li, Huanhuan Gavini, Elisabetta Patravale, Vandana B. Donnelly, Ryan F. |
Department | Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica |
Publication Date | 2023 |
Deposit Date | 2024-03-01 |
Published in |
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Abstract | Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing bacterial pathogen linked to superficial skin and soft tissue infections (SSTIs). Rifampicin (RIF), a potent antibiotic against systemic and localised ... Methicillin-resistant Staphylococcus aureus (MRSA) is a prevailing bacterial pathogen linked to superficial skin and soft tissue infections (SSTIs). Rifampicin (RIF), a potent antibiotic against systemic and localised staphylococcal infections, faces limitations due to its low solubility. This constraint hampers its therapeutic potential for MRSA-induced SSTIs. To address this, an advanced liposomal system was designed for efficient dermal RIF delivery. Rifampicin-loaded liposomes (LipoRIF) were embedded within polymeric dissolving microneedles (DMNs) to enable targeted intradermal drug delivery. A robust Design of Experiment (DoE) methodology guided the systematic preparation and optimisation of LipoRIF formulations. The optimal LipoRIF formulation integrated within polymeric DMNs. These LipoRIF-DMNs exhibited favourable mechanical properties and effective skin insertion characteristics. Notably, in vitro assays on skin deposition unveiled a transformative result – the DMN platform significantly enhanced LipoRIF deposition within the skin, surpassing LipoRIF dispersion alone. Moreover, LipoRIF-DMNs displayed minimal cytotoxicity toward cells. Encouragingly, rigorous in vitro antimicrobial evaluations demonstrated LipoRIF-DMNs' capacity to inhibit MRSA growth compared to the control group. LipoRIF-DMNs propose a potentially enhanced, minimally invasive approach to effectively manage SSTIs and superficial skin ailments stemming from MRSA infections. |
Funding agencies | Wellcome Trust |
Project ID. | WT094085MA |
Citation | Anjani, Q.K., Pandya, A.K., Demartis, S., Domínguez Robles, J., Moreno Castellanos, N., Li, H.,...,Donnelly, R.F. (2023). Liposome-loaded Polymeric Microneedles for Enhanced Skin Deposition of Rifampicin. International Journal of Pharmaceutics, 646, 123446. https://doi.org/10.1016/j.ijpharm.2023.123446. |
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