dc.creator | Delgado Requerey, Víctor | es |
dc.creator | Cejudo Fernández, Francisco Javier | es |
dc.creator | González García, María de la Cruz | es |
dc.date.accessioned | 2024-02-27T16:10:41Z | |
dc.date.available | 2024-02-27T16:10:41Z | |
dc.date.issued | 2023-05-03 | |
dc.identifier.citation | Delgado Requerey, V., Cejudo Fernández, F.J. y González García, M.d.l.C. (2023). The Functional Relationship between NADPH Thioredoxin Reductase C, 2-Cys Peroxiredoxins, and m-Type Thioredoxins in the Regulation of Calvin–Benson Cycle and Malate-Valve Enzymes in Arabidopsis. Antioxidants, 12 (5), 1041. https://doi.org/10.3390/antiox12051041. | |
dc.identifier.issn | 2076-3921 | es |
dc.identifier.uri | https://hdl.handle.net/11441/155673 | |
dc.description.abstract | The concerted regulation of chloroplast biosynthetic pathways and NADPH extrusion via malate valve depends on f and m thioredoxins (Trxs). The finding that decreased levels of the thiol-peroxidase 2-Cys peroxiredoxin (Prx) suppress the severe phenotype of Arabidopsis mutants lacking NADPH-dependent Trx reductase C (NTRC) and Trxs f uncovered the central function of the NTRC-2-Cys-Prx redox system in chloroplast performance. These results suggest that Trxs m are also regulated by this system; however, the functional relationship between NTRC, 2-Cys Prxs, and m-type Trxs is unknown. To address this issue, we generated Arabidopsis thaliana mutants combining deficiencies in NTRC, 2-Cys Prx B, Trxs m1, and m4. The single trxm1 and trxm4 mutants showed a wild-type phenotype, growth retardation being noticed only in the trxm1m4 double mutant. Moreover, the ntrc-trxm1m4 mutant displayed a more severe phenotype than the ntrc mutant, as shown by the impaired photosynthetic performance, altered chloroplast structure, and defective light-dependent reduction in the Calvin–Benson cycle and malate-valve enzymes. These effects were suppressed by the decreased contents of 2-Cys Prx, since the quadruple ntrc-trxm1m4-2cpb mutant displayed a wild-type-like phenotype. These results show that the activity of m-type Trxs in the light-dependent regulation of biosynthetic enzymes and malate valve is controlled by the NTRC-2-Cys-Prx system. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación PID2020-115156GB-100 | es |
dc.description.sponsorship | Junta de Andalucía P20_00567 | es |
dc.format | application/pdf | es |
dc.format.extent | 19 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.relation.ispartof | Antioxidants, 12 (5), 1041. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | 2-Cys peroxiredoxin | es |
dc.subject | Calvin–Benson cycle | es |
dc.subject | Chloroplast | es |
dc.subject | Malate valve | es |
dc.subject | NADPH-dependent Trx reductase (NTRC) | es |
dc.subject | Redox regulation | es |
dc.subject | Thioredoxin | es |
dc.title | The Functional Relationship between NADPH Thioredoxin Reductase C, 2-Cys Peroxiredoxins, and m-Type Thioredoxins in the Regulation of Calvin–Benson Cycle and Malate-Valve Enzymes in Arabidopsis | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | PID2020-115156GB-100 | es |
dc.relation.projectID | P20_00567 | es |
dc.relation.publisherversion | https://doi.org/10.3390/antiox12051041 | es |
dc.identifier.doi | 10.3390/antiox12051041 | es |
dc.journaltitle | Antioxidants | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 5 | es |
dc.publication.initialPage | 1041 | es |
dc.contributor.funder | Ministerio de Ciencia e Innovación (MICIN). España | es |
dc.contributor.funder | Junta de Andalucía | es |