Article
Increasing the Scalability of Toxin-Intein Orthogonal Combinations
Author/s | López Igual, Rocío
![]() ![]() ![]() ![]() ![]() ![]() ![]() Dorado Morales, Pedro Mazel, Didier |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2023-01-27 |
Deposit Date | 2024-02-23 |
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Abstract | Inteins are proteins embedded into host proteins from which they are excised in an autocatalytic reaction. Specifically, split inteins are separated into two independent fragments that reconstitute the host protein during ... Inteins are proteins embedded into host proteins from which they are excised in an autocatalytic reaction. Specifically, split inteins are separated into two independent fragments that reconstitute the host protein during the catalytic process. We recently developed a novel strategy for the specific killing of pathogenic and antibiotic resistant bacteria based on toxin-intein combinations. Bacterial type II toxin-antitoxin systems are protein modules in which the toxin can provoke cell death whereas the antitoxin inhibits toxin activity. Although our previous system was based on a split intein (iDnaE) and the CcdB toxin, we demonstrated that iDnaE is able to reconstitute four different toxins. To expand the applicability of our system by widening the repertoire of toxin-intein combinations for complex set-ups, we introduced a second intein, iDnaX, which was artificially split. We demonstrate that iDnaX is able to reconstitute the four toxins, and we manage to reduce its scar size to facilitate their use. In addition, we prove the orthogonality of both inteins (iDnaE and iDnaX) through a toxin reconstitution assay, thus opening the possibility for complex set-ups based on these toxin-intein modules. This could be used to develop specific antimicrobial and other biotechnological applications. |
Funding agencies | Centre national de la recherche scientifique (CNRS). France French Government Fondation pour la Recherche Medicale Ministerio de Ciencia e Innovación (MICIN). España |
Project ID. | CNRS-UMR 3525
![]() PLASWIRES 612146/FP7-FET ![]() ANR-10-LABX-62-IBEID ![]() EQU202103012569 ![]() PID2019-104784RJ ![]() BFU2017-88202-P ![]() |
Citation | López Igual, R., Dorado Morales, P. y Mazel, D. (2023). Increasing the Scalability of Toxin-Intein Orthogonal Combinations. ACS Synthetic Biology, 12 (2), 618-623. https://doi.org/10.1021/acssynbio.2c00477. |
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