Artículo
SUMO-activated Target Traps (SATTs) Enable the Identification of a Comprehensive E3-specific SUMO Proteome
Autor/es | Salas Lloret, Daniel
Jansen, Nicolette S. Nagamalleswari, Easa van der Meulen, Coen Gracheva, Ekaterina de Ru, Arnoud H. Otte, H. Anne Marie van Veelen, Peter A. Pichler, Andrea Goedhart, Joachim Vertegaal, Alfred C.O. González Prieto, Román |
Departamento | Universidad de Sevilla. Departamento de Biología Celular |
Fecha de publicación | 2023 |
Fecha de depósito | 2024-02-15 |
Publicado en |
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Premios | Premio Anual Publicación Científica Destacada de la US. Facultad de Biología |
Resumen | Ubiquitin and ubiquitin-like conjugation cascades consist of dedicated E1, E2, and E3 enzymes with E3s providing substrate specificity. Mass spectrometry–based approaches have enabled the identification of more than 6500 ... Ubiquitin and ubiquitin-like conjugation cascades consist of dedicated E1, E2, and E3 enzymes with E3s providing substrate specificity. Mass spectrometry–based approaches have enabled the identification of more than 6500 SUMO2/3 target proteins. The limited number of SUMO E3s provides the unique opportunity to systematically study E3 substrate wiring. We developed SUMO-activated target traps (SATTs) and systematically identified substrates for eight different SUMO E3s, PIAS1, PIAS2, PIAS3, PIAS4, NSMCE2, ZNF451, LAZSUL (ZNF451-3), and ZMIZ2. SATTs enabled us to identify 427 SUMO1 and 961 SUMO2/3 targets in an E3-specific manner. We found pronounced E3 substrate preference. Quantitative proteomics enabled us to measure substrate specificity of E3s, quantified using the SATT index. Furthermore, we developed the Polar SATTs web-based tool to browse the dataset in an interactive manner. Overall, we uncover E3-to-target wiring of 1388 SUMO substrates, highlighting unique and overlapping sets of substrates for eight different SUMO E3 ligases. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía Dutch Cancer Society European Research Council (ERC) Dutch Research Council Swiss National Science Foundation (SNFS) |
Identificador del proyecto | PID2021-122361NA-I00
EMERGIA20_00276 KWF-KIG 11367/2017-2 310913 724.016.003 310030_204583 |
Cita | Salas Lloret, D., Jansen, N.S., Nagamalleswari, E., van der Meulen, C., Gracheva, E., de Ru, A.H.,...,González Prieto, R. (2023). SUMO-activated Target Traps (SATTs) Enable the Identification of a Comprehensive E3-specific SUMO Proteome. Science Advances, 9 (31), eadh2073. https://doi.org/10.1126/sciadv.adh2073. |
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SUMO-activated target.pdf | 2.313Mb | [PDF] | Ver/ | |