Artículo
Functional Features of the Exopolysaccharide Extracts Produced by Lactiplantibacillus Strains Isolated from Table Olives
Autor/es | Lopez García, Elio
Marín Gordillo, Ana Sánchez Hidalgo, Marina Ávila Román, Francisco Javier Romero Gil, Verónica Bermúdez Oria, Alejandra Benítez Cabello, Antonio Garrido Fernández, Antonio Rodríguez Gómez, Francisco Arroyo López, Francisco Noé |
Departamento | Universidad de Sevilla. Departamento de Farmacología |
Fecha de publicación | 2024 |
Fecha de depósito | 2024-02-08 |
Publicado en |
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Resumen | This study evaluates the functional characteristics of the exopolysaccharide (EPS) extracts produced by various strains of Lactiplantibacillus pentosus (LPG1, 119, 13B4, and Lp13) and Lactiplantibacillus plantarum (Lp15) ... This study evaluates the functional characteristics of the exopolysaccharide (EPS) extracts produced by various strains of Lactiplantibacillus pentosus (LPG1, 119, 13B4, and Lp13) and Lactiplantibacillus plantarum (Lp15) isolated from table olives. None of the EPS crude extracts showed cytotoxicity when administered to THP-1 human macrophage cells at dosages ranging from 6.25 to 50 mug mL-1. Many exhibited anti-inflammatory properties (reduction of pro-inflammatory cytokines TNF-alpha and IL-6 production) and antioxidant activity (reduction of ROS%) when macrophages were stimulated with Escherichia coli lipopolysaccharide. Notably, the EPS extract produced by the L. pentosus LPG1 strain had the best results corroborated by western blot immune analysis for differential expression of COX-2, Nrf-2, and HO-1 proteins, with the most significant antioxidant and anti-inflammatory response observed at a dosage of 50 mug mL-1. Chemical analysis revealed that the EPS extract produced by this strain contains a heteropolymer composed of mannose (35.45%), glucose (32.99%), arabinose (17.93%), xylose (7.48%), galactose (4.03%), rhamnose (1.34%), and fucose (0.77%). Finally, we conducted response surface methodology to model the EPS extract production by L. pentosus LPG1 considering pH (3.48-8.52), temperature (16.59-33.41 °C) and salt concentration (0.03-8.77% NaCl) as independent variables. The model identified linear effects of salt and pH and quadratic effects of salt as significant terms. The maximum EPS extract production (566 mg L-1) in a synthetic culture medium (MRS) was achieved at pH 7.5, salt 7.0%, and a temperature of 20 °C. These findings suggest the potential for novel applications for the EPS produced by L. pentosus LPG1 as nutraceutical candidates for use in human diets. |
Agencias financiadoras | European Union (UE) Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | RTI2018–100883-B-I00
PRE2019-087812 FJC2019-040605- I PAIDI2020-00162 |
Cita | Lopez García, E., Marín Gordillo, A., Sánchez Hidalgo, M., Ávila Román, F.J., Romero Gil, V., Bermúdez Oria, A.,...,Arroyo López, F.N. (2024). Functional Features of the Exopolysaccharide Extracts Produced by Lactiplantibacillus Strains Isolated from Table Olives. Food & Funtion. https://doi.org/10.1039/D3FO04223E. |
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