Article
In Vitro Effects of Serotonin, Melatonin, and Other Related Indole Compounds on Amyloid-beta Kinetics and Neuroprotection
Author/s | Hornedo Ortega, Ruth
Da Costa, Grégory Cerezo López, Ana Belén Troncoso González, Ana María Richard, Tristan García Parrilla, María del Carmen |
Department | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Publication Date | 2018 |
Deposit Date | 2024-01-19 |
Published in |
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Awards | Premio Mensual Publicación Científica Destacada de la US. Facultad de Farmacia |
Abstract | Scope
Amyloid-β peptide is the main component of senile plaques in Alzheimer's disease. The inhibition of amyloid-β peptide assembly, the destabilization of amyloid-β peptide aggregates, and the decrease of its cytotoxicity ... Scope Amyloid-β peptide is the main component of senile plaques in Alzheimer's disease. The inhibition of amyloid-β peptide assembly, the destabilization of amyloid-β peptide aggregates, and the decrease of its cytotoxicity for the prevention of neuronal death are considered neuroprotective effects. In this work, the protective effects against amyloid-β peptide aggregation and cytotoxicity of eight indolic compounds are evaluated: tryptophan, tryptamine, serotonin, tryptophol, N-acetylserotonin, 3-indoleacetic acid, tryptophan ethyl ester, and melatonin. Methods and results Thioflavin T spectroscopic assay, transmission electron microscopy, western blotting, circular dichroism, NMR, cell viability (thiazolyl blue tetrazolium bromide assay), quantitative PCR, and heme oxygenase activity are used. Serotonin is the most effective compound for inhibiting amyloid-β peptide aggregation. Almost all the indolic compounds tested prevent amyloid-β peptide-induced and increase cell viability, being between 9 and 25%. Melatonin and serotonin are the most active. Moreover, serotonin increased the expression of SIRT-1 and 2, heat shock protein 70, and heme oxygenase activity, this being a possible mechanism underlying the observed neuroprotective effect. Conclusion Melatonin and other related indolic compounds, mainly serotonin, show an inhibitory and destabilizing effect on amyloid-β peptide fibril formation and they possess neuroprotective properties related to the vitagenes system. |
Funding agencies | Ministerio de Ciencia, Innovación y Universidades (MICINN). España |
Project ID. | AGL2013-47300-C3-2-R
AGL2016-77505-C3-2-R |
Citation | Hornedo Ortega, R., Da Costa, G., Cerezo López, A.B., Troncoso González, A.M., Richard, T. y García Parrilla, M.d.C. (2018). In Vitro Effects of Serotonin, Melatonin, and Other Related Indole Compounds on Amyloid-beta Kinetics and Neuroprotection. Molecular Nutrition & Food Research, 62 (3), 1700383. https://doi.org/10.1002/mnfr.201700383. |
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