Article
Characterization of bioactive compounds in defatted hempseed (Cannabis sativa L.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes
Author/s | Rea Martínez, Julio
Montserrat de la Paz, Sergio ![]() ![]() ![]() ![]() ![]() ![]() ![]() Puerta Vázquez, Rocío de la ![]() ![]() ![]() ![]() ![]() ![]() ![]() García Giménez, María Dolores Fernández Arche, María de los Ángeles |
Department | Universidad de Sevilla. Departamento de Farmacología Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología |
Publication Date | 2020 |
Deposit Date | 2024-01-18 |
Published in |
|
Abstract | Hempseed (Cannabis sativa L.) has beneficial impact on human health mainly because of its wide variability of bioactive compounds. However, many of them are not fully characterized yet. In this work, hempseed was defatted ... Hempseed (Cannabis sativa L.) has beneficial impact on human health mainly because of its wide variability of bioactive compounds. However, many of them are not fully characterized yet. In this work, hempseed was defatted and through a bio-guided studied, two fractions (F03 and F05) with the highest content of phenols, flavonoids and antioxidant capacity were selected. Fractions were chemically analyzed by UHPLC HRMS/MS. The anti-inflammatory capacities of these compounds were evaluated on human monocytes using flow cytometry, RT-qPCR and Elisa procedures. A high amount of phenolic compounds were identified, with the major compound being: N-trans-caffeoyltyramine (6.36 mg g−1 in F05 and 1.28 mg g−1 in F03). Both, F03 and F05 significantly reduced the inflammatory competence of LPS-treated human primary monocytes, decreasing TNF-α and IL-6 gene expression and secretion. These findings indicate that in the defatted fraction of the hempseed there are a wide number of compounds with beneficial potential to prevent and treat inflammatory disorders, as well as other processes caused by oxidative stress. |
Citation | Rea Martínez, J., Montserrat de la Paz, S., Puerta Vázquez, R.d.l., García Giménez, M.D. y Fernández Arche, M.d.l.Á. (2020). Characterization of bioactive compounds in defatted hempseed (Cannabis sativa L.) by UHPLC-HRMS/MS and anti-inflammatory activity in primary human monocytes. Food & Function, 11 (5), 4057-4066. https://doi.org/10.1039/d0fo00066c. |
Files | Size | Format | View | Description |
---|---|---|---|---|
10.1039d0fo00066c.pdf | 1.291Mb | ![]() | View/ | Versión aceptada |
This item appears in the following collection(s)
This document is protected by intellectual and industrial property rights. Without prejudice to existing legal exemptions, its reproduction, distribution, public communication or transformation is prohibited without the authorization of the rights holder, unless otherwise indicated.