dc.creator | Rocha de Lossada, Carlos | es |
dc.creator | Mazzotta, Cosimo | es |
dc.creator | Gabrielli, Federico | es |
dc.creator | Papa, Filomena Tiziana | es |
dc.creator | Gómez Huertas, Carmen | es |
dc.creator | García López, Celia | es |
dc.creator | Urbinati, Facundo | es |
dc.creator | Rachwani-Anil, Rahul | es |
dc.creator | García Lorente, María | es |
dc.creator | Sánchez González, José María | es |
dc.creator | Rechichi, Miguel | es |
dc.creator | Rubegni, Giovanni | es |
dc.creator | Borroni, Davide | es |
dc.date.accessioned | 2023-11-22T16:40:00Z | |
dc.date.available | 2023-11-22T16:40:00Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Rocha de Lossada, C., Mazzotta, C., Gabrielli, F., Papa, F.T., Gómez Huertas, C., García López, C.,...,Borroni, D. (2023). Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study. Journal of Clinical Medicine, 12 (9), 6354. https://doi.org/10.3390/jcm12196354. | |
dc.identifier.issn | 2077-0383 | es |
dc.identifier.uri | https://hdl.handle.net/11441/151385 | |
dc.description.abstract | In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development. | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.relation.ispartof | Journal of Clinical Medicine, 12 (9), 6354. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Keratoconus | es |
dc.subject | Metagenomics | es |
dc.subject | Microbiota | es |
dc.subject | Validation-study | es |
dc.title | Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Física de la Materia Condensada | es |
dc.relation.publisherversion | https://doi.org/10.3390/jcm12196354 | es |
dc.identifier.doi | 10.3390/jcm12196354 | es |
dc.journaltitle | Journal of Clinical Medicine | es |
dc.publication.volumen | 12 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 6354 | es |