Artículo
Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study
Autor/es | Rocha de Lossada, Carlos
Mazzotta, Cosimo Gabrielli, Federico Papa, Filomena Tiziana Gómez Huertas, Carmen García López, Celia Urbinati, Facundo Rachwani-Anil, Rahul García Lorente, María Sánchez González, José María Rechichi, Miguel Rubegni, Giovanni Borroni, Davide |
Departamento | Universidad de Sevilla. Departamento de Física de la Materia Condensada |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-11-22 |
Publicado en |
|
Resumen | In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular ... In the field of Ophthalmology, the mNGS 16S rRNA sequencing method of studying the microbiota and ocular microbiome is gaining more and more weight in the scientific community. This study aims to characterize the ocular microbiota of patients diagnosed with keratoconus who have not undergone any prior surgical treatment using the mNGS 16S rRNA sequencing method. Samples of naïve keratoconus patients were collected with an eNAT with 1 mL of Liquid Amies Medium (Copan Brescia, Italy), and DNA was extracted and analyzed with 16S NGS. The microbiota analysis showed a relative abundance of microorganisms at the phylum level in each sample collected from 38 patients with KC and 167 healthy controls. A comparison between healthy control and keratoconus samples identified two genera unique to keratoconus, Pelomonas and Ralstonia. Our findings suggest that alterations in the microbiota may play a role in the complex scenario of KC development. |
Cita | Rocha de Lossada, C., Mazzotta, C., Gabrielli, F., Papa, F.T., Gómez Huertas, C., García López, C.,...,Borroni, D. (2023). Ocular Surface Microbiota in Naïve Keratoconus: A Multicenter Validation Study. Journal of Clinical Medicine, 12 (9), 6354. https://doi.org/10.3390/jcm12196354. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Ocular Surface Microbiota.pdf | 2.136Mb | [PDF] | Ver/ | |