Artículo
Potential Oestrogenic Effects (Following the OECD Test Guideline 440) and Thyroid Dysfunction Induced by Pure Cyanotoxins (Microcystin-LR, Cylindrospermopsin) in Rats
Autor/es | Casas Rodríguez, Antonio
Moyano, Rosario Molina Hernández, Verónica Cameán Fernández, Ana María Jos Gallego, Ángeles Mencía |
Departamento | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-11-20 |
Publicado en |
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Premios | Premio Mensual Publicación Científica Destacada de la US. Facultad de Farmacia |
Resumen | Potential endocrine-disrupting properties of cyanotoxins, such as microcystin-LR (MC-LR) and cylindrospermopsin (CYN) are of concern due to their increasing occurrence, the scarcity of reports on the topic (particularly ... Potential endocrine-disrupting properties of cyanotoxins, such as microcystin-LR (MC-LR) and cylindrospermopsin (CYN) are of concern due to their increasing occurrence, the scarcity of reports on the topic (particularly for CYN) and the impact of human's health at different levels. Thus, this work performed for the first time the uterotrophic bioassay in rats, following the Organization for Economic Cooperation and Development (OECD) Test Guideline 440, to explore the oestrogenic properties of CYN and MC-LR (75, 150, 300 μg/kg b.w./day) in ovariectomized (OVX) rats. Results revealed neither changes in the wet and blotted uterus weights nor in the morphometric study of uteri. Moreover, among the steroid hormones analysed in serum, the most remarkable effect was the dose-dependent increase in progesterone (P) levels in rats exposed to MC-LR. Additionally, a histopathology study of thyroids and serum levels of thyroids hormones were determined. Tissue affectation (follicular hypertrophy, exfoliated epithelium, hyperplasia) was observed, as well as increased T3 and T4 levels in rats exposed to both toxins. Taken together, these results point out that CYN and MC-LR are not oestrogenic compounds at the conditions tested in the uterotrophic assay in OVX rats, but, however, thyroid disruption effects cannot be discarded. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | PRE 2020-094412
P18-RTJ-1956 |
Cita | Casas Rodríguez, A., Moyano, R., Molina Hernández, V., Cameán Fernández, A.M. y Jos Gallego, Á.M. (2023). Potential Oestrogenic Effects (Following the OECD Test Guideline 440) and Thyroid Dysfunction Induced by Pure Cyanotoxins (Microcystin-LR, Cylindrospermopsin) in Rats. Environmental Research, 226, 115671. https://doi.org/10.1016/j.envres.2023.115671. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Potential oestrogenic effects.pdf | 6.258Mb | [PDF] | Ver/ | |