dc.creator | Cebadero Domínguez, Óscar | es |
dc.creator | Casas Rodríguez, Antonio | es |
dc.creator | Puerto Rodríguez, María | es |
dc.creator | Cameán Fernández, Ana María | es |
dc.creator | Jos Gallego, Ángeles Mencía | es |
dc.date.accessioned | 2023-11-17T16:58:41Z | |
dc.date.available | 2023-11-17T16:58:41Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Cebadero Domínguez, Ó., Casas Rodríguez, A., Puerto Rodríguez, M., Cameán Fernández, A.M. y Jos Gallego, Á.M. (2023). In Vitro Safety Assessment of Reduced Graphene Oxide in Human Monocytes and T Cells.. Environmental Research, 232, 116356. https://doi.org/10.1016/j.envres.2023.116356. | |
dc.identifier.issn | 0013-9351 | es |
dc.identifier.issn | 1096-0953 | es |
dc.identifier.uri | https://hdl.handle.net/11441/151031 | |
dc.description.abstract | Considering the increase in the use of graphene derivatives in different fields, the environmental and human exposure to these materials is likely, and the potential consequences are not fully elucidated. This study is focused on the human immune system, as this plays a key role in the organism's homeostasis. In this sense, the cytotoxicity response of reduced graphene oxide (rGO) was investigated in monocytes (THP-1) and human T cells (Jurkat). A mean effective concentration (EC50-24 h) of 121.45 ± 11.39 μg/mL and 207.51 ± 21.67 μg/mL for cytotoxicity was obtained in THP-1 and Jurkat cells, respectively. rGO decreased THP-1 monocytes differentiation at the highest concentration after 48 h of exposure. Regarding the inflammatory response at genetic level, rGO upregulated IL-6 in THP-1 and all cytokines tested in Jurkat cells after 4 h of exposure. At 24 h, IL-6 upregulation was maintained, and a significant decrease of TNF-α gene expression was observed in THP-1 cells. Moreover, TNF-α, and INF-γ upregulation were maintained in Jurkat cells. With respect to the apoptosis/necrosis, gene expression was not altered in THP-1 cells, but a down regulation of BAX and BCL-2 was observed in Jurkat cells after 4 h of exposure. These genes showed values closer to negative control after 24 h. Finally, rGO did not trigger a significant release of any cytokine at any exposure time assayed. In conclusion, our data contributes to the risk assessment of this material and suggest that rGO has an impact on the immune system whose final consequences should be further investigated. | es |
dc.description.sponsorship | Fondo Europeo de Desarrollo Regional US-1259106 | es |
dc.description.sponsorship | Junta de Andalucía P18-RT-1993 | es |
dc.format | application/pdf | es |
dc.format.extent | 8 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Environmental Research, 232, 116356. | |
dc.relation.isreferencedby | Cebadero Domínguez, Ó., Casas Rodríguez, A.,...,Jos Gallego, Á.M. (2022). Study the immunotoxic effect of reduced graphene [Dataset]. idUS (Depósito de Investigación de la Universidad de Sevilla). https://doi.org/10.12795/11441/152721 | |
dc.rights | Atribución-NoComercial 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | * |
dc.subject | Cytokines | es |
dc.subject | Differentiation | es |
dc.subject | Jurkat cell | es |
dc.subject | Reduced graphene oxide | es |
dc.subject | THP-1 cells | es |
dc.title | In Vitro Safety Assessment of Reduced Graphene Oxide in Human Monocytes and T Cells. | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal | es |
dc.relation.projectID | US-1259106 | es |
dc.relation.projectID | P18-RT-1993 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.envres.2023.116356 | es |
dc.identifier.doi | 10.1016/j.envres.2023.116356 | es |
dc.journaltitle | Environmental Research | es |
dc.publication.volumen | 232 | es |
dc.publication.initialPage | 116356 | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.description.awardwinning | Premio Mensual Publicación Científica Destacada de la US. Facultad de Farmacia | |