Article
Immunomodulatory Effects of Cylindrospermopsin in Human T Cells and Monocytes
Author/s | Casas Rodríguez, Antonio
Cebadero Domínguez, Óscar Puerto Rodríguez, María Cameán Fernández, Ana María Jos Gallego, Ángeles Mencía |
Department | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Publication Date | 2023 |
Deposit Date | 2023-11-17 |
Published in |
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Abstract | Cylindrospermopsin (CYN) is a cyanotoxin with an increasing occurrence, and therefore it is important to elucidate its toxicity profile. CYN has been classified as a cytotoxin, although the scientific literature has already ... Cylindrospermopsin (CYN) is a cyanotoxin with an increasing occurrence, and therefore it is important to elucidate its toxicity profile. CYN has been classified as a cytotoxin, although the scientific literature has already revealed that it affects a wide range of organs and systems. However, research on its potential immunotoxicity is still limited. Thus, this study aimed to evaluate the impact of CYN on two human cell lines representative of the immune system: THP-1 (monocytes) and Jurkat (lymphocytes). CYN reduced cell viability, leading to mean effective concentrations (EC50 24 h) of 6.00 ± 1.04 µM and 5.20 ± 1.20 µM for THP-1 and Jurkat cells, respectively, and induced cell death mainly by apoptosis in both experimental models. Moreover, CYN decreased the differentiation of monocytes to macrophages after 48 h of exposure. In addition, an up-regulation of the mRNA expression of different cytokines, such as interleukin (IL) 2, IL-8, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (INF-γ), was also observed mainly after 24 h exposure in both cell lines. However, only an increase in TNF-α in THP-1 supernatants was observed by ELISA. Overall, these results suggest the immunomodulatory activity of CYN in vitro. Therefore, further research is required to evaluate the impact of CYN on the human immune system. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España Acciones Marie Sklodowska-Curie (MSCA) Universidad de Sevilla |
Project ID. | PID 2019-104890RB-I0
PRE 2020-094412 823860 CITIUS–VIIPPIT-2023-I.3 |
Referenced by | Casas Rodríguez, A., Cebadero Domínguez, Ó.,...,Jos Gallego, Á.M.. Study the immunotoxic effect of cylindrospermopsin [Dataset]. idUS (Depósito de Investigación de la Universidad de Sevilla). https://doi.org/10.12795/11441/152718 |
Citation | Casas Rodríguez, A., Cebadero Domínguez, Ó., Puerto Rodríguez, M., Cameán Fernández, A.M. y Jos Gallego, Á.M. (2023). Immunomodulatory Effects of Cylindrospermopsin in Human T Cells and Monocytes. Toxins, 15 (4), 301. https://doi.org/10.3390/toxins15040301. |
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