Datos de Investigación (Nutrición y Bromatología, Toxicología y Medicina Legal )
URI permanente para esta colecciónhttps://hdl.handle.net/11441/150658
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Dataset The Production of Bioactive Hydroxytyrosol in Fermented Beverages: The Role of Must Composition and a Genetically Modified Yeast Strain [Dataset](2024-04) González Ramírez, Marina; Gallardo Fernández, Marta; Cerezo López, Ana Belén; Bisquert, Ricardo; Valero, Eva; Troncoso González, Ana María; García Parrilla, María del Carmen; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; González Ramírez, MarinaThe dataset include the raw data of the analysys of HPLC-MS/MS for tyrosol and hydroxytyrosol. The samples were from fermented synthetic must by the yeas strain Red Fruit in different conditions of YAN (210 mg/L and 300 mg/L) and different glucose conditions (100 g/L y 240 g/L).Dataset Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites [Dataset](2020) Gallardo Fernández, Marta; Cerezo López, Ana Belén; Hornedo Ortega, Ruth; Troncoso González, Ana María; García Parrilla, María del Carmen; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Cerezo López, Ana Belén; Ministerio de Ciencia, Innovación y Universidades (MICINN). España; Universidad de Sevilla. AGR 167: Derivados de la uvaEl dasaset incluye los datos brutos de los experimentos western blot y ELISA realizados en el trabajo titulado "Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites". También se incluyen las medias y las desviaciones estándar.
Dataset Study the immunotoxic effect of reduced graphene [Dataset](2023-12-20) Cebadero Domínguez, Óscar; Casas Rodríguez, Antonio; Puerto Rodríguez, María; Cameán Fernández, Ana María; Jos Gallego, Ángeles Mencía; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Jos Gallego, Ángeles Mencía; Cebadero Domínguez, Óscar; Jos Gallego, Ángeles Mencía; Junta de Andalucía; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. CTS358: ToxicologíaThe cytotoxicity response of reduced graphene oxide (rGO) was investigated in monocytes (THP-1) and human T cells (Jurkat). A mean effective concentration (EC50-24 h) of 121.45 ± 11.39 μg/mL and 207.51 ± 21.67 μg/mL for cytotoxicity was obtained in THP-1 and Jurkat cells, respectively. rGO decreased THP-1 monocytes differentiation at the highest concentration after 48 h of exposure. Regarding the inflammatory response at genetic level, changes (up and downregulations) were observed depending on the cell line, the exposure time and the interleukins investigated. Apoptosis/ necrosis genes expression was not altered in THP-1 cells but in Jurkat cells BAX and BCL-2 were downregulated after 4h. Finally, rGO did not trigger a significant release of any cytokine at any exposure time assayed. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, gene expression and interleukin leakage.Dataset Study the immunotoxic effect of cylindrospermopsin [Dataset](2023-12-20) Casas Rodríguez, Antonio; Cebadero Domínguez, Óscar; Puerto Rodríguez, María; Cameán Fernández, Ana María; Jos Gallego, Ángeles Mencía; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Cameán Fernández, Ana María; Casas Rodríguez, Antonio; Puerto Rodríguez, María; Ministerio de Ciencia e Innovación (MICIN). España; Universidad de Sevilla. CTS358: ToxicologíaCylindrospermopsin (CYN) is a cyanotoxin with an increasing occurrence, and therefore it is important to elucidate its toxicity profile. CYN has been classified as a cytotoxin, although the scientific literature has already revealed that it affects a wide range of organs and systems. However, research on its potential immunotoxicity is still limited. Thus, this study aimed to evaluate the impact of CYN on two human cell lines representative of the immune system: THP-1 (monocytes) and Jurkat (lymphocytes). CYN reduced cell viability, leading to mean effective concentrations (EC50 24 h) of 6.00± 1.04 µM and 5.20± 1.20 µM for THP-1 and Jurkat cells, respectively, and induced cell death mainly by apoptosis in both experimental models. Moreover, CYN decreased the differentiation of monocytes to macrophages after 48 h of exposure. In addition, an up-regulation of the mRNA expression of different cytokines, such as interleukin (IL) 2, IL-8, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (INF-ɣ), was also observed mainly after 24 h exposure in both cell lines. However, only an increase in TNF-_ in THP-1 supernatants was observed by ELISA. Overall, these results suggest the immunomodulatory activity of CYN in vitro. Therefore, further research is required to evaluate the impact of CYN on the human immune system. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, cell death mechanisms (apoptosis/necrosis), gene expression and interleukin leakage.Dataset Genotoxicity evaluation of graphene in vitro assays [dataset](2023-12-20) Cebadero Domínguez, Óscar; Medrano Padial, Concepción; Puerto Rodríguez, María; Sánchez Ballester, Soraya; Cameán Fernández, Ana María; Jos Gallego, Ángeles Mencía; Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal; Puerto Rodríguez, María; Cebadero Domínguez, Óscar; Puerto Rodríguez, María; Junta de Andalucía; European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER); Universidad de Sevilla. CTS358: ToxicologíaThe aim of this study was to assess the potential mutagenicity and genotoxicity of graphene oxide (GO) and reduced-graphene oxide (rGO) following the recommendations of the European Food Safety Authority (EFSA). Thus, the mouse lymphoma assay (MLA) and the micronucleus test (MN) were performed in L5178YTk ± cells, and the Caco-2 cells were used for the standard and modified comet assays. The results indicated that GO (0–250 μg/mL) was not mutagenic in the MLA. However, rGO revealed mutagenic activity from 250 μg/mL and 125 μg/mL after 4h and 24h of exposure, respectively. In the MN test, negative results were obtained for both compounds at the concentrations assayed (0–250 μg/mL) for GO/rGO. Moreover, no DNA strand breaks, or oxidative DNA damage were detected in Caco-2 cells exposed to GO (0–250 μg/mL) and rGO (0–176.3 μg/mL for 24h and 0–166.5 μg/mL for 48h). Dataset contains the numerical values of the results obtained regarding to MLA assay 4 and 24 h, MN test 24h and comet assay 24 and 48h.