Article
Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer
Author/s | Vázquez Carretero, María Dolores
![]() ![]() ![]() ![]() ![]() ![]() ![]() García Miranda, Pablo ![]() ![]() ![]() ![]() ![]() ![]() ![]() Balda, María S. Matter, Karl Ilundáin Larrañeta, María Anunciación Ana Peral Rubio, María José ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Fisiología |
Publication Date | 2021 |
Deposit Date | 2023-07-05 |
Published in |
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Abstract | We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane ... We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalization indicating that its membrane location requires Dab2. In mice, we found that 3 days of dextran sulfate sodium-induced colitis increased Dab2/E-cadherin colocalization, which was decreased as colitis progressed to 6 and 9 days. In agreement, Dab2/E-cadherin colocalization increased in human mild and severe ulcerative colitis and in polyps, being reduced in colon adenocarcinomas, which even showed epithelial Dab2 absence and E-cadherin delocalization. Epithelial Dab2 decrement preceded that of E-cadherin. We suggest that Dab2, by inhibiting E-cadherin internalization, stabilizes adherens junctions, and its absence from the epithelium may contribute to development of colon inflammation and cancer. |
Funding agencies | Junta de Andalucía |
Project ID. | CTS 5884
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Citation | Vázquez Carretero, M.D., García Miranda, P., Balda, M.S., Matter, K., Ilundáin Larrañeta, M.A.A. y Peral Rubio, M.J. (2021). Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer. Journal of Cellular Physiology, 236 (2), 1083-1093. https://doi.org/10.1002/jcp.29917. |
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