Article
Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm
Author/s | Revilla Gonzalez, Gonzalo
Varela Pérez, Lourdes María Ruiz de Azua-López, Zaida Amaya-Villar, Rosario Rosa Pezzotti, Maria Castro, María José Ureña López, Juan González-Montelongo, María del Carmen Castellano Orozco, Antonio Gonzalo |
Department | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica |
Publication Date | 2023 |
Deposit Date | 2023-06-27 |
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Abstract | Aneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The ... Aneurysmal subarachnoid hemorrhage (aSAH) is a neurovascular disease produced by extravasation of blood to the subarachnoid space after rupture of the cerebral vessels. After bleeding, the immune response is activated. The role of peripheral blood mononuclear cells (PBMCs) in this response is a current subject of research. We have analysed the changes in PBMCs of patients with aSAH and their interaction with the endothelium, focusing on their adhesion and the expression of adhesion molecules. Using an in vitro adhesion assay, we observed that the adhesion of PBMCs of patients with aSAH is increased. Flow cytometry analysis shows that monocytes increased signifcantly in patients, especially in those who developed vasospasm (VSP). In aSAH patients, the expression of CD162, CD49d, CD62L and CD11a in T lymphocytes and of CD62L in monocytes increased. However, the expression of CD162, CD43, and CD11a decreased in monocytes. Furthermore, monocytes from patients who developed arteriographic VSP had lower expression of CD62L. In conclusion, our results confrm that after aSAH, monocyte count and adhesion of PBMCs increase, especially in patients with VSP, and that the expression of several adhesion molecules is altered. These observations can help predict VSP and to improve the treatment of this pathology. |
Funding agencies | Universidad de Sevilla/ CBUA |
Project ID. | SAF2017- 89474-R |
Citation | Revilla Gonzalez, G., Varela Pérez, L.M., Ruiz de Azua-López, Z., Amaya-Villar, R., Rosa Pezzotti, M., Castro, M.J.,...,Castellano Orozco, A.G. (2023). Changes in adhesion and the expression of adhesion molecules in PBMCs after aneurysmal subarachnoid hemorrhage: relation to cerebral vasospasm. https://doi.org/10.1007/s12975-023-01136-6. |
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