Article
Acyclic Diterpene Phytol from Hemp Seed Oil (Cannabis sativa L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages
Author/s | Claro Cala, Carmen María
Grao Cruces, Elena Toscano Sánchez, María del Rocío Millán-Linares, María del Carmen Montserrat de la Paz, Sergio Martín Rubio, María Esther |
Department | Universidad de Sevilla. Departamento de Farmacología, Pediatría y Radiología Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología Universidad de Sevilla. Departamento de Biología Celular |
Publication Date | 2022 |
Deposit Date | 2023-06-20 |
Published in |
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Abstract | Seeds from non-drug varieties of hemp (Cannabis sativa L.) have been used for traditional
medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil
(HSO) exerts anti-inflammatory ... Seeds from non-drug varieties of hemp (Cannabis sativa L.) have been used for traditional medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil (HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes and human macrophages derived from circulating monocytes were used to evaluate both plasticity and anti-inflammatory effects of phytol from HSO at 10–100 mM using FACS analysis, ELISA, and RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14+CD16++ monocyte phenotype and toward macrophage M2 (CD200Rhigh and MRC-1high), as well as to reduce the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages. In addition, our study may help to understand the mechanisms by which phytol from HSO contributes to the constant and progressive plasticity of the human monocyte-macrophage linage. |
Citation | Claro Cala, C.M., Grao Cruces, E., Toscano Sánchez, M.d.R., Millán-Linares, M.d.C., Montserrat de la Paz, S. y Martín Rubio, M.E. (2022). Acyclic Diterpene Phytol from Hemp Seed Oil (Cannabis sativa L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages. Foods, 11 (15), 2366. https://doi.org/10.3390/foods11152366. |
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