Article
Up-Regulation of the Nrf2/HO-1 Antioxidant Pathway in Macrophages by an Extract from a New Halophilic Archaea Isolated in Odiel Saltworks
Author/s | Ávila Román, Francisco Javier
Gómez Villegas, Patricia Carvalho, Carla de Vigara, Javier Motilva Sánchez, Virginia León, Rosa Talero Barrientos, Elena Mª |
Department | Universidad de Sevilla. Departamento de Farmacología |
Publication Date | 2023 |
Deposit Date | 2023-05-15 |
Published in |
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Abstract | : The production of reactive oxygen species (ROS) plays an important role in the progression of
many inflammatory diseases. The search for antioxidants with the ability for scavenging free radicals
from the body cells ... : The production of reactive oxygen species (ROS) plays an important role in the progression of many inflammatory diseases. The search for antioxidants with the ability for scavenging free radicals from the body cells that reduce oxidative damage is essential to prevent and treat these pathologies. Haloarchaea are extremely halophilic microorganisms that inhabit hypersaline environments, such as saltworks or salt lakes, where they have to tolerate high salinity, and elevated ultraviolet (UV) and infrared radiations. To cope with these extreme conditions, haloarchaea have developed singular mechanisms to maintain an osmotic balance with the medium, and are endowed with unique compounds, not found in other species, with bioactive properties that have not been fully explored. This study aims to assess the potential of haloarchaea as a new source of natural antioxidant and anti-inflammatory agents. A carotenoid-producing haloarchaea was isolated from Odiel Saltworks (OS) and identified on the basis of its 16S rRNA coding gene sequence as a new strain belonging to the genus Haloarcula. The Haloarcula sp. OS acetone extract (HAE) obtained from the biomass contained bacterioruberin and mainly C18 fatty acids, and showed potent antioxidant capacity using ABTS assay. This study further demonstrates, for the first time, that pretreatment with HAE of lipopolysaccharide (LPS)-stimulated macrophages results in a reduction in ROS production, a decrease in the pro-inflammatory cytokines TNF-α and IL-6 levels, and up-regulation of the factor Nrf2 and its target gene heme oxygenase-1 (HO-1), supporting the potential of the HAE as a therapeutic agent in the treatment of oxidative stress-related inflammatory diseases. |
Funding agencies | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Agencia Estatal de Investigación. España Junta de Andalucía Universidad de Sevilla Fundação para a Ciência e a Tecnologia. Portugal Associate Laboratory Institute for Health and Bioeconomy (i4HB). Portugal Universidad de Huelva |
Project ID. | US-1380844
UHU-1257518 PID2019-110438RBC22-AEI/FEDER I+D+i-JA-PAIDI-Retos 2020-PY20 USE-VIPPI UIDB/04565/2020 UIDP/04565/2020 i4HB LA/P/0140/2020 |
Citation | Ávila Román, F.J., Gómez Villegas, P., Carvalho, C.d., Vigara, J., Motilva Sánchez, V., León, R. y Talero Barrientos, E.M. (2023). Up-Regulation of the Nrf2/HO-1 Antioxidant Pathway in Macrophages by an Extract from a New Halophilic Archaea Isolated in Odiel Saltworks. Antioxidants, 12 (5), 1080. https://doi.org/10.3390/antiox12051080. |
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