dc.creator | Ortiz, Tamara | es |
dc.creator | Villanueva-Paz, Marina | es |
dc.creator | Díaz-Parrado, Eduardo | es |
dc.creator | Illanes, Matilde | es |
dc.creator | Fernández-Rodríguez, Ana | es |
dc.creator | Sánchez-Alcázar, José A. | es |
dc.creator | Miguel Rodríguez, Manuel de | es |
dc.date.accessioned | 2023-03-06T13:52:21Z | |
dc.date.available | 2023-03-06T13:52:21Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Ortiz, T., Villanueva-Paz, M., Díaz-Parrado, E., Illanes, M., Fernández-Rodríguez, A., Sánchez-Alcázar, J.A. y Miguel Rodríguez, M.d. (2017). Amitriptyline down-regulates coenzyme Q10 biosynthesis in lung cancer cells. European Journal of Pharmacology, 797, 75-82. https://doi.org/10.1016/j.ejphar.2017.01.017. | |
dc.identifier.issn | 0014-2999 | es |
dc.identifier.uri | https://hdl.handle.net/11441/143176 | |
dc.description.abstract | Amitriptyline, a tricyclic antidepressant, has been proposed as an antitumoral drug in oxidative therapy. Its pro-apoptotic
effects, mediated by high reactive oxygen species generation, have been already described. In this study we analysed the
effect of amitriptyline on the biosynthesis of coenzyme Q10 (CoQ), an essential component for electron transport and a
potent membrane antioxidant involved in redox signaling. We treated H460 cells, a non-small-cell lung cancer cell line,
with amitriptyline and we analysed CoQ levels by HPLC and CoQ biosynthesis rate, as well as the enzymes involved in
CoQ biosynthesis by real-time PCR and Western blot. Amitriptyline treatment induced a dose-dependent decrease in CoQ
levels in tumor cells. CoQ decreased levels were associated with down-regulation of the expression of COQ4 gene, as
well as decreased Coq4 and Coq6 protein levels. Our Vndings suggest that the effect of amitriptyline on CoQ biosynthesis
highlights the potential of this drug for antitumoral oxidative therapy. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | European Journal of Pharmacology, 797, 75-82. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Amitriptyline | es |
dc.subject | Coenzyme Q10 | es |
dc.subject | Oxidative stress | es |
dc.subject | Antioxidants | es |
dc.subject | Oxidative therapy | es |
dc.title | Amitriptyline down-regulates coenzyme Q10 biosynthesis in lung cancer cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.ejphar.2017.01.017 | es |
dc.identifier.doi | 10.1016/j.ejphar.2017.01.017 | es |
dc.journaltitle | European Journal of Pharmacology | es |
dc.publication.volumen | 797 | es |
dc.publication.initialPage | 75 | es |
dc.publication.endPage | 82 | es |