Article
Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review
Author/s | Ruiz Duque, Borja
Bañuls, Lucía Reinoso Arija, Rocío Carrasco Hernández, Laura Caballero Eraso, Candelaria Dasí, Francisco López-Campos Bodineau, José Luis |
Department | Universidad de Sevilla. Departamento de Medicina |
Publication Date | 2021-10-31 |
Deposit Date | 2022-11-10 |
Published in |
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Abstract | Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic ... Background: The study of hematic concentrations of alpha1 antitrypsin (AAT) is currently one step in the diagnosis of AAT deficiency. To try to clarify the relevance of the laboratory techniques, we carried out a systematic review of the literature. Methods: Studies evaluating the quantification of AAT in peripheral blood were searched in PubMed in July 2021. The selection criteria included (1) any type of study design that included a quantification of AAT in peripheral blood; (2) studies written in English or Spanish; (3) studies evaluating human beings; and (4) studies involving adults. Results: Out of 207 studies, the most frequently used techniques were nephelometry (43.9%), followed by ELISA (19.8%) and turbidimetry (13.5%). Altogether, 182 (87.9%) cases expressed their results in units of gram, while 16 (7.7%) articles expressed them in units of mole. Only 2.9% articles referred to the standard used, 43.5% articles indicated the commercial kit used, and 36.2% indicated the analyzer used. Conclusions: The technical aspects of these determinations are not always reported in the literature. Journals should be attentive to these technical requirements and ensure that they are included in the works in which AAT is determined in order to ensure a correct interpretation of the study findings. |
Funding agencies | Sociedad Valenciana Neumologia 112/2016 SEPAR; ACIF/2019/231 GVA |
Project ID. | 112/2016 SEPAR
ACIF/2019/231 GVA |
Citation | Ruiz Duque, B., Bañuls, L., Reinoso Arija, R., Carrasco Hernández, L., Caballero Eraso, C., Dasí, F. y López-Campos Bodineau, J.L. (2021). Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review. Journal of Clinical Medicine (JCM), 10 (21), 5132. https://doi.org/10.3390/jcm10215132. |
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