dc.creator | Casarrubios, Marta | es |
dc.creator | Cruz-Bermúdez, Alberto | es |
dc.creator | Nadal, Ernest | es |
dc.creator | Insa, Amelia | es |
dc.creator | del Rosario García Campelo, María | es |
dc.creator | Lázaro, Martín | es |
dc.creator | Dómine, Manuel | es |
dc.creator | Caro, Reyes Bernabé | es |
dc.creator | Provencio, Mariano | es |
dc.date.accessioned | 2022-09-27T14:12:31Z | |
dc.date.available | 2022-09-27T14:12:31Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Casarrubios, M., Cruz-Bermúdez, A., Nadal, E., Insa, A., del Rosario García Campelo, M., Lázaro, M.,...,Provencio, M. (2021). Pretreatment tissue TCR repertoire evenness is associated with complete pathologic response in patients with NSCLC receiving neoadjuvant chemoimmunotherapy. Clinical Cancer Research, 27 (21), 5878-5890. | |
dc.identifier.issn | 1078-0432 | es |
dc.identifier.issn | 1557-3265 | es |
dc.identifier.uri | https://hdl.handle.net/11441/137402 | |
dc.description.abstract | Purpose: Characterization of the T-cell receptor (TCR) reper-
toire may be a promising source for predictive biomarkers of
pathologic response to immunotherapy in locally advanced non–
small cell lung cancer (NSCLC).
Experimental Design: In this study, next-generation TCR
sequencing was performed in peripheral blood and tissue sam-
ples of 40 patients with NSCLC, before and after neoadjuvant
chemoimmunotherapy (NADIM clinical trial, NCT03081689),
considering their complete pathologic response (CPR) or non-
CPR. Beyond TCR metrics, tissue clones were ranked by their
frequency and spatiotemporal evolution of top 1% clones was
determined.
Results: We have found a positive association between an uneven
TCR repertoire in tissue samples at diagnosis and CPR at surgery.
Moreover, TCR most frequently ranked clones (top 1%) present in
diagnostic biopsies occupied greater frequency in the total clonal
space of CPR patients, achieving an AUC ROC to identify CPR
patients of 0.967 (95% confidence interval, 0.897–1.000; P ¼ 0.001),
and improving the results of PD-L1 tumor proportion score (TPS;
AUC ¼ 0.767; P ¼ 0.026) or tumor mutational burden (TMB; AUC
¼ 0.550; P ¼ 0.687). Furthermore, tumors with high pretreatment
top 1% clonal space showed similar immune cell populations but a
higher immune reactive gene expression profile. Finally, the selec-
tive expansion of pretreatment tissue top 1% clones in peripheral
blood of CPR patients suggests also a peripheral immunosurveil-
lance, which could explain the high survival rate of these patients.
Conclusions: We have identified two parameters derived from
TCR repertoire analysis that could outperform PD-L1 TPS
and TMB as predictive biomarkers of CPR after neoadjuvant
chemoimmunotherapy, and unraveled possible mechanisms of
CPR involving enhanced tumor immunogenicity and peripheral
immunosurveillance. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 | es |
dc.language.iso | eng | es |
dc.publisher | American Association for Cancer Research | es |
dc.relation.ispartof | Clinical Cancer Research, 27 (21), 5878-5890. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | TCR Repertoire | es |
dc.subject | NSCLC | es |
dc.subject | Tissue | es |
dc.subject | Chemoimmunotherapy | es |
dc.title | Pretreatment tissue TCR repertoire evenness is associated with complete pathologic response in patients with NSCLC receiving neoadjuvant chemoimmunotherapy | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.publisherversion | http://doi.org/10.1158/1078-0432.CCR-21-1200 | es |
dc.identifier.doi | 10.1158/1078-0432.CCR-21-1200 | es |
dc.journaltitle | Clinical Cancer Research | es |
dc.publication.volumen | 27 | es |
dc.publication.issue | 21 | es |
dc.publication.initialPage | 5878 | es |
dc.publication.endPage | 5890 | es |