Article
Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning
Author/s | Mayoral González, Isabel
Bevilacqua, Elisa Gómez, Gorka Hmadcha, Abdelkrim González-Loscertales, Ignacio Reina Romo, Esther Sotelo, Julio Domínguez, Antonia Pérez Alcántara, Pedro Smani, Younes González Puertas, Patricia Isabel Méndez, Ana Uribe, Sergio Smani Hajami, Tarik Ordóñez, Antonio Valverde Pérez, Israel |
Department | Universidad de Sevilla. Departamento de Ingeniería Mecánica y Fabricación Universidad de Sevilla. Departamento de Farmacología, Pediatría y Radiología Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica |
Publication Date | 2022-03 |
Deposit Date | 2022-08-26 |
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Abstract | Three-dimensional (3D) engineered cardiovascular tissues have shown great promise to replace damaged structures. Specifically, tissue engineering vascular grafts (TEVG) have the potential to replace biological and synthetic ... Three-dimensional (3D) engineered cardiovascular tissues have shown great promise to replace damaged structures. Specifically, tissue engineering vascular grafts (TEVG) have the potential to replace biological and synthetic grafts. We aimed to design an in-vitro patient-specific patch based on a hybrid 3D print combined with vascular smooth muscle cells (VSMC) differentiation. Based on the medical images of a 2 months-old girl with aortic arch hypoplasia and using computational modelling, we evaluated the most hemodynamically efficient aortic patch surgical repair. Using the designed 3D patch geometry, the scaffold was printed using a hybrid fused deposition modelling (FDM) and electrospinning techniques. The scaffold was seeded with multipotent mesenchymal stem cells (MSC) for later maturation to derived VSMC (dVSMC). The graft showed adequate resistance to physiological aortic pressure (burst pressure 101 ± 15 mmHg) and a porosity gradient ranging from 80 to 10 μm allowing cells to infiltrate through the entire thickness of the patch. The bio-scaffolds showed good cell viability at days 4 and 12 and adequate functional vasoactive response to endothelin-1. In summary, we have shown that our method of generating patient-specific patch shows adequate hemodynamic profile, mechanical properties, dVSMC infiltration, viability and functionality. This innovative 3D biotechnology has the potential for broad application in regenerative medicine and potentially in heart disease prevention. |
Project ID. | PI20/00467
PI17/01409 PT20/00069 Beca ‘Menudos Corazones 2020’ PID2019-104084GB-C22 ICN2021_004 #11200481 #1181057 |
Citation | Mayoral González, I., Bevilacqua, E., Gómez, G., Hmadcha, A., González-Loscertales, I., Reina Romo, E.,...,Valverde Pérez, I. (2022). Tissue engineered in-vitro vascular patch fabrication using hybrid 3D printing and electrospinning. Materials Today Bio, 14, 100252. |
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