Article
Grb2 and Its Apoptotic Isoform Grb3-3 Associate with Heterogeneous Nuclear Ribonucleoprotein C, and These Interactions Are Modulated by Poly(U) RNA
Author/s | Romero Portillo, Francisco
![]() ![]() ![]() ![]() ![]() ![]() ![]() Ramos Morales, Francisco ![]() ![]() ![]() ![]() ![]() ![]() ![]() Domínguez, África Ríos Sánchez, Rosa María Schweighoffer, Fabien Tocqué, Bruno Pintor Toro, José Antonio Fischer, Siegmund Tortolero García, María Dolores |
Department | Universidad de Sevilla. Departamento de Microbiología |
Publication Date | 1998 |
Deposit Date | 2022-07-19 |
Published in |
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Abstract | Grb2 is an adaptor molecule comprising one Src homology (SH) 2 and two SH3 domains. This protein has a natural isoform named Grb3-3 with a deletion within the SH2 domain. Numerous evidence points to a functional connection ... Grb2 is an adaptor molecule comprising one Src homology (SH) 2 and two SH3 domains. This protein has a natural isoform named Grb3-3 with a deletion within the SH2 domain. Numerous evidence points to a functional connection between SH2- and SH3-containing proteins and molecules implicated in RNA biogenesis. In this context, we have examined the binding of Grb2 and Grb3-3 to heterogeneous nuclear ribonucleoprotein (hnRNP) C. By the use of an in vivo genetic approach and through in vitroexperiments, we furnish evidence that both Grb2 and Grb3-3 interact with hnRNP C proteins. Subcellular fractionation studies clearly show that Grb2 is partially localized in the nucleus. In addition, coimmunoprecipitation experiments demonstrate that Grb2·hnRNP C complexes exist in intact hematopoietic cells. The carboxyl-terminal SH3 domains of Grb2 and Grb3-3 are primarily responsible for the association with hnRNP C. However, although the proline-rich motif of hnRNP C is involved in the interaction with Grb2, it is not in the binding to Grb3-3. Furthermore, poly(U) RNA inhibits the association of Grb2 with hnRNP C, whereas it enhances the interaction between Grb3-3 and hnRNP C. These findings suggest that the Grb2/Grb3-3-hnRNP C interactions might fulfill different biological functions. |
Citation | Romero Portillo, F., Ramos Morales, F., Domínguez, Á., Ríos Sánchez, R.M., Schweighoffer, F., Tocqué, B.,...,Tortolero García, M.D. (1998). Grb2 and Its Apoptotic Isoform Grb3-3 Associate with Heterogeneous Nuclear Ribonucleoprotein C, and These Interactions Are Modulated by Poly(U) RNA. Journal of Biological Chemistry, 273 (13), 7776-7781. |
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