Artículo
Carbohydrate-DNA interactions at G-quadruplexes: Folding and stability changes by attaching sugars at the 5′-End
Autor/es | Gómez Pinto, Irene
Vengut Climent, Empar Lucas Rodríguez, Ricardo Aviñó, Anna Eritja, Ramón González, Carlos Morales, Juan Carlos |
Departamento | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica |
Fecha de publicación | 2013 |
Fecha de depósito | 2022-07-12 |
Publicado en |
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Resumen | Quadruplex DNA structures are attracting an enormous interest in many areas of chemistry, ranging from chemical biology, supramolecular chemistry to nanoscience. We have prepared carbohydrate-DNA conjugates containing the ... Quadruplex DNA structures are attracting an enormous interest in many areas of chemistry, ranging from chemical biology, supramolecular chemistry to nanoscience. We have prepared carbohydrate-DNA conjugates containing the oligonucleotide sequences of G-quadruplexes (thrombin binding aptamer (TBA) and human telomere (TEL)), measured their thermal stability and studied their structure in solution by using NMR and molecular dynamics. The solution structure of a fucose-TBA conjugate shows stacking interactions between the carbohydrate and the DNA G-tetrad in addition to hydrogen bonding and hydrophobic contacts. We have also shown that attaching carbohydrates at the 5′-end of a quadruplex telomeric sequence can alter its folding topology. These results suggest the possibility of modulating the folding of the G-quadruplex by linking carbohydrates and have clear implications in molecular recognition and the design of new G-quadruplex ligands. |
Agencias financiadoras | Ministerio de Ciencia, Innovación y Universidades (MICINN). España European Union (UE) Generalitat de Catalunya Instituto de Salud Carlos III |
Identificador del proyecto | CTQ2009–13705
CTQ2010–20451 CTQ2010–21567-C02–02 MP0802 2009/SGR/208 CB06 01 0019 |
Cita | Gómez Pinto, I., Vengut Climent, E., Lucas Rodríguez, R., Aviñó, A., Eritja, R., González, C. y Morales, J.C. (2013). Carbohydrate-DNA interactions at G-quadruplexes: Folding and stability changes by attaching sugars at the 5′-End. Chemistry - A European Journal, 19 (6), 1920-1927. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Carbohydrate–DNA Interactions.pdf | 4.499Mb | [PDF] | Ver/ | |