dc.creator | Leo, Simone Di | es |
dc.creator | Marni, Stefano | es |
dc.creator | Plata Ramos, Carlos Alberto | es |
dc.creator | Fraccia, Tommaso P. | es |
dc.creator | Smith, Gregory P. | es |
dc.creator | Maritan, Amos | es |
dc.creator | Suweis, Samir | es |
dc.creator | Bellini, Tommaso | es |
dc.date.accessioned | 2022-06-08T10:40:33Z | |
dc.date.available | 2022-06-08T10:40:33Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Leo, S.D., Marni, S., Plata Ramos, C.A., Fraccia, T.P., Smith, G.P., Maritan, .,...,Bellini, T. (2022). Pairing statistics and melting of random DNA oligomers: Finding your partner in superdiverse environments. Plos Computational Biology, 18 (4), e1010051. | |
dc.identifier.issn | 1553-7358 | es |
dc.identifier.uri | https://hdl.handle.net/11441/134210 | |
dc.description.abstract | Understanding of the pairing statistics in solutions populated by a large number of distinct solute species with mutual interactions is a challenging topic, relevant in modeling the complexity of real biological systems. Here we describe, both experimentally and theoretically, the formation of duplexes in a solution of random-sequence DNA (rsDNA) oligomers of length L = 8, 12, 20 nucleotides. rsDNA solutions are formed by 4L distinct molecular species, leading to a variety of pairing motifs that depend on sequence complementarity and range from strongly bound, fully paired defectless helices to weakly interacting mismatched duplexes. Experiments and theory coherently combine revealing a hybridization statistics characterized by a prevalence of partially defected duplexes, with a distribution of type and number of pairing errors that depends on temperature. We find that despite the enormous multitude of inter-strand interactions, defectless duplexes are formed, involving a fraction up to 15% of the rsDNA chains at the lowest temperatures. Experiments and theory are limited here to equilibrium conditions. | es |
dc.format | application/pdf | es |
dc.format.extent | 20 p. | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | Plos Computational Biology, 18 (4), e1010051. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Pairing statistics and melting of random DNA oligomers: Finding your partner in superdiverse environments | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Física Atómica, Molecular y Nuclear | es |
dc.relation.publisherversion | https://dx.doi.org/10.1371/journal.pcbi.1010051 | es |
dc.identifier.doi | 10.1371/journal.pcbi.1010051 | es |
dc.journaltitle | Plos Computational Biology | es |
dc.publication.volumen | 18 | es |
dc.publication.issue | 4 | es |
dc.publication.endPage | e1010051 | es |