Artículo
The Interactions of Cyanobacterial Cytochromec6 and Cytochrome f, Characterized by NMR
Autor/es | Crowley, Peter B.
Díaz Quintana, Antonio Jesús Molina Heredia, Fernando Publio Nieto, Pedro Sutter, Martin Haehnel, Wolfgang Rosa Acosta, Miguel Ángel de la Ubbínk, Marcellus |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2002 |
Fecha de depósito | 2022-05-31 |
Publicado en |
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Resumen | During oxygenic photosynthesis, cytochromec6 shuttles electrons between the membrane-bound complexes cytochrome bf and photosystem I. Complex formation between Phormidium laminosum cytochromef and cytochrome c6 from ... During oxygenic photosynthesis, cytochromec6 shuttles electrons between the membrane-bound complexes cytochrome bf and photosystem I. Complex formation between Phormidium laminosum cytochromef and cytochrome c6 from bothAnabaena sp. PCC 7119 and Synechococcus elongatus has been investigated by nuclear magnetic resonance spectroscopy. Chemical-shift perturbation analysis reveals a binding site on Anabaena cytochrome c6, which consists of a predominantly hydrophobic patch surrounding the heme substituent, methyl 5. This region of the protein was implicated previously in the formation of the reactive complex with photosytem I. In contrast to the results obtained for Anabaena cytochromec6, there is no evidence for specific complex formation with the acidic cytochrome c6 fromSynechococcus. This remarkable variability between analogous cytochromes c6 supports the idea that different organisms utilize distinct mechanisms of photosynthetic intermolecular electron transfer. |
Agencias financiadoras | European Commission (EC) Ministerio de Ciencia Y Tecnología (MCYT). España Junta de Andalucía |
Identificador del proyecto | HPRN-CT-1999-00095
BMC2000-0444 CVI-0198 |
Cita | Crowley, P.B., Díaz Quintana, A.J., Molina Heredia, F.P., Nieto, P., Sutter, M., Haehnel, W.,...,Ubbínk, M. (2002). The Interactions of Cyanobacterial Cytochromec6 and Cytochrome f, Characterized by NMR. Journal of Biological Chemistry, 277 (50), 48685-48689. |
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