Artículo
Structure of the Complex between Plastocyanin and Cytochrome f from the Cyanobacterium Nostoc sp. PCC 7119 as Determined by Paramagnetic NMR
Autor/es | Díaz Moreno, Irene
Díaz Quintana, Antonio Jesús Rosa Acosta, Miguel Ángel de la Ubbink, Marcellus |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2005 |
Fecha de depósito | 2022-05-26 |
Publicado en |
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Resumen | The complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc has been characterized by NMR spectroscopy. The binding constant is 16 mm–1, and the lifetime of the complex is much less than 10 ms. ... The complex between cytochrome f and plastocyanin from the cyanobacterium Nostoc has been characterized by NMR spectroscopy. The binding constant is 16 mm–1, and the lifetime of the complex is much less than 10 ms. Intermolecular pseudo-contact shifts observed for the plastocyanin amide nuclei, caused by the heme iron, as well as the chemical-shift perturbation data were used as the sole experimental restraints to determine the orientation of plastocyanin relative to cytochrome f with a precision of 1.3 Å. The data show that the hydrophobic patch surrounding tyrosine 1 in cytochrome f docks the hydrophobic patch of plastocyanin. Charge complementarities are found between the rims of the respective recognition sites of cytochrome f and plastocyanin. Significant differences in the relative orientation of both proteins are found between this complex and those previously reported for plants and Phormidium, indicating that electrostatic and hydrophobic interactions are balanced differently in these complexes. |
Agencias financiadoras | European Commission (EC) Ministerio de Educación, Cultura y Deporte (MECD). España Ministerio de Ciencia y Tecnología (MCYT). España Junta de Andalucía Netherlands Organization for Scientific Research |
Identificador del proyecto | HPRN-CT-1999-00095
AP2000-2937 BMC2003-00458 CVI-0198 700.52.425 |
Cita | Díaz Moreno, I., Díaz Quintana, A.J., Rosa Acosta, M.Á.d.l. y Ubbink, M. (2005). Structure of the Complex between Plastocyanin and Cytochrome f from the Cyanobacterium Nostoc sp. PCC 7119 as Determined by Paramagnetic NMR. Journal of Biological Chemistry, 280 (19), 18908-18915. |
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