Article
Proteomic insight into the effects of the Salmonella ubiquitin ligase SlrP on host cells
Author/s | Cordero Alba, Mar
García Gómez, Juan José Aguilera Herce, Julia Ramos Morales, Francisco ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2016 |
Deposit Date | 2022-03-21 |
Published in |
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Abstract | The virulence of the human and animal pathogen Salmonella enterica serovar Typhimurium is dependent on two type III secretion systems. These systems translocate proteins called effectors into eukaryotic host cells. SlrP ... The virulence of the human and animal pathogen Salmonella enterica serovar Typhimurium is dependent on two type III secretion systems. These systems translocate proteins called effectors into eukaryotic host cells. SlrP is a Salmonella type III secretion effector with ubiquitin ligase activity. Here, we used two complementary proteomic approaches, two-dimensional gel electrophoresis and iTRAQ (isobaric tags for relative and absolute quantification) to study the consequences of the presence of SlrP in human epithelial cells. We identified 37 proteins that were differentially expressed in HeLa cells expressing slrP compared to control cells. Microarray analysis revealed that more than a half of differentially expressed proteins did not show changes in the transcriptome, suggesting post-transcriptional regulation. A gene ontology overrepresentation test carried out on the differentially expressed proteins revealed enrichment of ontology terms related to several types of junctions mediating adhesion in epithelial cells. Consistently, slrP-transfected cells showed defects in migration and adhesion. Our results suggest that the modification of cell–cell interaction ability of the host could be one of the final consequences of the action of SlrP during an infection. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Junta de Andalucía |
Project ID. | SAF2010-15015
![]() SAF2013-46229-R ![]() P08-CVI-03487 ![]() |
Citation | Cordero Alba, M., García Gómez, J.J., Aguilera Herce, J. y Ramos Morales, F. (2016). Proteomic insight into the effects of the Salmonella ubiquitin ligase SlrP on host cells. Biochemical and Biophysical Research Communications, 472 (3), 539-544. |
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