dc.creator | Macher, Hada | es |
dc.creator | Suárez Artacho, Gonzalo | es |
dc.creator | Guerrero Montávez, Juan Miguel | es |
dc.creator | Gómez Bravo, Miguel Ángel | es |
dc.creator | Álvarez Gómez, Sara | es |
dc.creator | Bernal Bellido, Carmen | es |
dc.creator | Domínguez Pascual, Inmaculada | es |
dc.creator | Rubio Calvo, Amalia Macarena | es |
dc.date.accessioned | 2022-02-08T07:19:06Z | |
dc.date.available | 2022-02-08T07:19:06Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | https://hdl.handle.net/11441/129734 | |
dc.description.abstract | Background: Health assessment of the transplanted organ is very important due to the relationship of long-term survival of organ transplant recipients and health organ maintenance. Nowadays, the measurement of cell-free DNA from grafts in the circulation of transplant recipients has been considered a potential biomarker of organ rejection or transplant associated complications in an attempt to replace or reduce liver biopsy. However, methods developed to date are expensive and extremely time-consuming. Our approach was to measure the SRY gene, as a male organ biomarker, in a setting of sex-mismatched female recipients of male donor organs.
Methods: Cell-free DNA quantization of the SRY gene was performed by real-time quantitative PCR beforehand, at the moment of transplantation during reperfusion (day 0) and during the stay at the intensive care unit. Beta-globin cell-free DNA levels, a general cellular damage marker, were also quantified.
Results: Beta-globin mean values of patients, who accepted the graft without any complications during the first week after surgery, diminished from day 0 until patient stabilization. This decrease was not so evident in patients who suffered some kind of post-transplantation complications. All patients showed an increase in SRY levels at day 0, which decreased during hospitalization. Different complications that did not compromise donated organs showed increased beta-globin levels but no SRY gene levels. However, when a donated organ was damaged the patients exhibited high levels of both genes.
Conclusion: Determination of a SRY gene in a female recipient's serum is a clear and specific biomarker of donated organs and may give us important information about graft health in a short period of time by a non-expensive technique. This approach may permit clinicians to maintain a close follow up of the transplanted patient. | es |
dc.format | application/pdf | es |
dc.format.extent | 18 | es |
dc.language.iso | eng | es |
dc.publisher | PLOS | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Chromosomes, Human, Y / genetics | es |
dc.subject | DNA / blood | es |
dc.subject | DNA / isolation & purification | es |
dc.subject | Genetic Markers | es |
dc.subject | Genomics | es |
dc.subject | Liver Transplantation | es |
dc.subject | Organ Specificity | es |
dc.subject | Sex-Determining Region Y Protein / blood | es |
dc.subject | Transplant Recipients | es |
dc.subject | beta-Globins / metabolism | es |
dc.subject | SRY protein, human | es |
dc.title | Monitoring of Transplanted Liver Health by Quantification of Organ-Specific Genomic Marker in Circulating DNA from Receptor | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.identifier.doi | 10.1371/journal.pone.0113987 | es |
dc.contributor.group | Universidad de Sevilla. CTS160: Neuroendocrinología Molecular | es |
dc.contributor.group | Universidad de Sevilla. CTS664: Cirugía Avanzada y Trasplantes. Terapia Celular y Bioingeniería Aplicada a la Cirugía | es |
dc.journaltitle | PLoS ONE | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 12 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 18 | es |