Article
A new method for the simultaneous determination of cyanotoxins (Microcystins and Cylindrospermopsin) in mussels using SPE-UPLC-MS/MS
Author/s | Díez-Quijada Jiménez, Leticia
![]() ![]() ![]() ![]() ![]() ![]() Guzmán Guillén, Remedios ![]() ![]() ![]() ![]() ![]() ![]() Cătunescu, Giorgiana M. Campos, Alexandre Vasconcelos, Vitor Jos Gallego, Ángeles Mencía ![]() ![]() ![]() ![]() ![]() ![]() Cameán Fernández, Ana María ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Publication Date | 2020 |
Deposit Date | 2021-12-09 |
Published in |
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Abstract | The aim of this study was to optimize the extraction conditions of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR) and Cylindrospermopsin (CYN) simultaneously from mussels by using response surface ... The aim of this study was to optimize the extraction conditions of Microcystin-LR (MC-LR), Microcystin-RR (MC-RR), Microcystin-YR (MC-YR) and Cylindrospermopsin (CYN) simultaneously from mussels by using response surface methodology (RSM) and to validate the method by a dual solid phase extraction (SPE) system combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The optimal parameters were: 90% MeOH (% v/v) for the extraction, a solvent/sample ratio of 75 and 15% MeOH in the extract before loading onto SPE. Mussels were spiked at 10; 37.5 and 75 ng g−1 fresh weight (f.w) of the 4 toxins, showing linear ranges of 0.5–75 ng g−1 f.w; low values for the limits of detection (0.01–0.39 ng g−1 f.w.) and quantification (0.23–0.40 ng g−1 f.w.); acceptable recoveries (70.37–114.03%) and relative standard deviation (%RSDIP) values (2.61–13.73%). The method was successfully applied to edible mussels exposed to cyanobacterial extracts under laboratory conditions, and it could allow the monitoring of these cyanotoxins in environmental mussel samples. |
Funding agencies | Ministerio de Economía y Competitividad (MINECO). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Fundação para a Ciência e a Tecnologia. Portugal European Commission (EC) |
Project ID. | AGL 2015-64558-R
![]() PTDC/ASPPES/31762/2017 ![]() UID/Multi/04423/2013 ![]() |
Citation | Díez-Quijada Jiménez, L., Guzmán Guillén, R., Cătunescu, G.M., Campos, A., Vasconcelos, V., Jos Gallego, Á.M. y Cameán Fernández, A.M. (2020). A new method for the simultaneous determination of cyanotoxins (Microcystins and Cylindrospermopsin) in mussels using SPE-UPLC-MS/MS. Environmental Research, 185, 109284. |
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10.1016_j.envres.2020.109284.pdf | 507.5Kb | ![]() | View/ | Postprint |