Artículo
Mutagenicity and genotoxicity assessment of a new biopreservative product rich in Enterocin AS-48
Autor/es | Cascajosa Lira, Antonio
Prieto Ortega, Ana Isabel Puerto Rodríguez, María Baños, Alberto Valdivia, E. Jos Gallego, Ángeles Mencía Cameán Fernández, Ana María |
Departamento | Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal |
Fecha de publicación | 2020 |
Fecha de depósito | 2021-12-09 |
Publicado en |
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Resumen | A biopreservative derived from the fermentation of a dairy byproduct by Enterococcus faecalis UGRA10 strains being developed. This product possesses a strong and wide antibacterial spectrum mainly due to the presence of ... A biopreservative derived from the fermentation of a dairy byproduct by Enterococcus faecalis UGRA10 strains being developed. This product possesses a strong and wide antibacterial spectrum mainly due to the presence of Enterocin AS-48 in its composition. To assess its potential as food additive, the mutagenicicity and genotoxicity has been assayed by means of the bacterial reverse-mutation assay in Salmonella typhimurium TA97A, TA98, TA100, TA102, TA1535 strains (Ames test, OECD 471, 2020) and the micronucleus test (MN) (OECD 487, 2016) in L5178Y/Tk ± cells. The results in the Ames test after exposure to the byproduct (6.75–100 μg/plate) with absence and presence of the metabolic activation system from rat liver (S9 fraction), revealed not mutagenicity at the conditions tested. For the MN test, the exposition to five enterocin AS-48 concentrations (0.2–1 μg/μl) was tested in the absence and presence of S9 fraction, with no evidence of genotoxicity. Negative results in the mutagenicity and genotoxicity assays point out the good safety profile of the byproduct and support its use as additive. Further toxicological studies are required before its approval and commercial application. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Junta de Andalucía |
Identificador del proyecto | RTC-2017-6199- 2
AT 2017–5323 |
Cita | Cascajosa Lira, A., Prieto Ortega, A.I., Puerto Rodríguez, M., Baños, A., Valdivia, E., Jos Gallego, Á.M. y Cameán Fernández, A.M. (2020). Mutagenicity and genotoxicity assessment of a new biopreservative product rich in Enterocin AS-48. Food and Chemical Toxicology, 146, 111846. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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10.1016_j.fct.2020.111846.pdf | 379.5Kb | [PDF] | Ver/ | |