dc.creator | Sessa, Gaetana | es |
dc.creator | Gómez González, Belén María | es |
dc.creator | Silva, Sonia | es |
dc.creator | Pérez Calero, Carmen | es |
dc.creator | Beaurepere, Romane | es |
dc.creator | Barroso Ceballos, Sonia Inés | es |
dc.creator | Martineau, Sylvain | es |
dc.creator | Martin, Charlotte A. | es |
dc.creator | Ehlén, Åsa | es |
dc.creator | Martínez, Juan S. | es |
dc.creator | Lombard, Bérangére | es |
dc.creator | Loew, Damarys | es |
dc.creator | Vagner, Stéphan | es |
dc.creator | Aguilera López, Andrés | es |
dc.creator | Carreira, Aura | es |
dc.date.accessioned | 2021-05-27T16:17:35Z | |
dc.date.available | 2021-05-27T16:17:35Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Sessa, G., Gómez González, B.M., Silva, S., Pérez Calero, C., Beaurepere, R., Barroso Ceballos, S.I.,...,Carreira, A. (2021). BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair. EMBO Journal, 40 (7), e106018. | |
dc.identifier.issn | 0261-4189 | es |
dc.identifier.issn | 1460-2075 | es |
dc.identifier.uri | https://hdl.handle.net/11441/110909 | |
dc.description.abstract | The BRCA2 tumor suppressor is a DNA double-strand break (DSB) repair factor essential for maintaining genome integrity. BRCA2-deficient cells spontaneously accumulate DNA-RNA hybrids, a known source of genome instability. However, the specific role of BRCA2 on these structures remains poorly understood. Here we identified the DEAD-box RNA helicase DDX5 as a BRCA2-interacting protein. DDX5 associates with DNA-RNA hybrids that form in the vicinity of DSBs, and this association is enhanced by BRCA2. Notably, BRCA2 stimulates the DNA-RNA hybrid-unwinding activity of DDX5 helicase. An impaired BRCA2-DDX5 interaction, as observed in cells expressing the breast cancer variant BRCA2-T207A, reduces the association of DDX5 with DNA-RNA hybrids, decreases the number of RPA foci, and alters the kinetics of appearance of RAD51 foci upon irradiation. Our findings are consistent with DNA-RNA hybrids constituting an impediment for the repair of DSBs by homologous recombination and reveal BRCA2 and DDX5 as active players in their removal. | es |
dc.description.sponsorship | Agence National de Recherche ANR-17-CE12-0016 | es |
dc.description.sponsorship | Institut National du Cancer INCa- DGOS_8706 | es |
dc.description.sponsorship | European Research Council ERC2014 AdG669898 TARLOOP | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2016-75058-P | es |
dc.format | application/pdf | es |
dc.format.extent | 25 p. | es |
dc.language.iso | eng | es |
dc.publisher | Wiley-Blackwell | es |
dc.relation.ispartof | EMBO Journal, 40 (7), e106018. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | BRCA2 | es |
dc.subject | DNA double-strand breaks | es |
dc.subject | DNA-RNA hybrids | es |
dc.subject | Homologous recombination | es |
dc.subject | R-loops | es |
dc.title | BRCA2 promotes DNA-RNA hybrid resolution by DDX5 helicase at DNA breaks to facilitate their repair | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.projectID | ANR-17-CE12-0016 | es |
dc.relation.projectID | INCa- DGOS_8706 | es |
dc.relation.projectID | ERC2014 AdG669898 TARLOOP | es |
dc.relation.projectID | BFU2016-75058-P | es |
dc.relation.publisherversion | https://doi.org/10.15252/embj.2020106018 | es |
dc.identifier.doi | 10.15252/embj.2020106018 | es |
dc.journaltitle | EMBO Journal | es |
dc.publication.volumen | 40 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | e106018 | es |