dc.creator | Carrascal Moreno, María Livia | es |
dc.creator | Gorton, Ella | es |
dc.creator | Pardillo Díaz, Ricardo | es |
dc.creator | Pérez García, Patricia | es |
dc.creator | Gómez Oliva, Ricardo | es |
dc.creator | Nuñez Abades, Pedro Antonio | es |
dc.date.accessioned | 2021-03-03T13:19:43Z | |
dc.date.available | 2021-03-03T13:19:43Z | |
dc.date.issued | 2020-12 | |
dc.identifier.citation | Carrascal Moreno, M.L., Gorton, E., Pardillo Díaz, R., Pérez García, P., Gómez Oliva, R. y Nuñez Abades, P.A. (2020). Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons. Antioxidants, 9 (12), 1307. | |
dc.identifier.issn | 2076-3921 | es |
dc.identifier.uri | https://hdl.handle.net/11441/105602 | |
dc.description.abstract | Oxidative stress is one of the main proposed mechanisms involved in neuronal degeneration.
To evaluate the consequences of oxidative stress on motor cortex pyramidal neurons during postnatal
development, rats were classified into three groups: Newborn (P2–P7); infantile (P11–P15); and young
adult (P20–P40). Oxidative stress was induced by 10 µM of cumene hydroperoxide (CH) application.
In newborn rats, using the whole cell patch-clamp technique in brain slices, no significant modifications
in membrane excitability were found. In infantile rats, the input resistance increased and rheobase
decreased due to the blockage of GABAergic tonic conductance. Lipid peroxidation induced by
CH resulted in a noticeable increase in protein-bound 4-hidroxynonenal in homogenates in only
infantile and young adult rat slices. Interestingly, homogenates of newborn rat brain slices showed the
highest capacity to respond to oxidative stress by dramatically increasing their glutathione and free
thiol content. This increase correlated with a time-dependent increase in the glutathione reductase
activity, suggesting a greater buffering capacity of newborn rats to resist oxidative stress. Furthermore,
pre-treatment of the slices with glutathione monoethyl ester acted as a neuroprotector in pyramidal
neurons of infantile rats. We conclude that during maturation, the vulnerability to oxidative stress in
rat motor neurons increases with age. | es |
dc.description.sponsorship | Ministerio de Ciencia, Innovación y Universidades españo-RTI2018-099908-B-C21 | es |
dc.description.sponsorship | Programa Operativo FEDER 2014-2020 y Departamento de Economía, Conocimiento, Empresa y Universidad de la Junta de Andalucía-FEDER-UCA18-106647 | es |
dc.format | application/pdf | es |
dc.format.extent | 21 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Antioxidants, 9 (12), 1307. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | oxidative stress | es |
dc.subject | motor cortex | es |
dc.subject | glutathione levels in the brain | es |
dc.subject | postnatal development | es |
dc.subject | membrane excitability | es |
dc.subject | amyotrophic lateral sclerosis | es |
dc.title | Age-Dependent Vulnerability to Oxidative Stress of Postnatal Rat Pyramidal Motor Cortex Neurons | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | RTI2018-099908-B-C21 | es |
dc.relation.projectID | FEDER-UCA18-106647 | es |
dc.relation.publisherversion | https://doi.org/10.3390/antiox9121307 | es |
dc.identifier.doi | 10.3390/antiox9121307 | es |
dc.journaltitle | Antioxidants | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 12 | es |
dc.publication.initialPage | 1307 | es |
dc.contributor.funder | Ministerio de Ciencia, Innovación y Universidades (MICINN). España | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.contributor.funder | Junta de Andalucía | es |