dc.creator | Ayerbe Algaba, Rafael | es |
dc.creator | Gil Marqués, María Luisa | es |
dc.creator | Jiménez-Mejías, Manuel Enrique | es |
dc.creator | Sánchez-Encinales, Viviana | es |
dc.creator | Parra Millán, Raquel | es |
dc.creator | Pachón Ibáñez, María Eugenia | es |
dc.creator | Pachón Díaz, Jerónimo | es |
dc.creator | Smani, Younes | es |
dc.date.accessioned | 2020-10-02T18:02:37Z | |
dc.date.available | 2020-10-02T18:02:37Z | |
dc.date.issued | 2018-10-03 | |
dc.identifier.citation | Ayerbe Algaba, R., Gil Marqués, M.L., Jiménez-Mejías, M.E., Sánchez-Encinales, V., Parra Millán, R., Pachón Ibáñez, M.E.,...,Smani, Y. (2018). Synergistic Activity of Niclosamide in Combination With Colistin Against Colistin-Susceptible and Colistin-Resistant Acinetobacter baumannii and Klebsiella pneumoniae. Frontiers in cellular and infection microbiology, 8 | |
dc.identifier.issn | 2235-2988 | es |
dc.identifier.uri | https://hdl.handle.net/11441/101684 | |
dc.description.abstract | Colistin is among the few antibiotics effective against multidrug-resistant Acinetobacter
baumannii and Klebsiella pneumoniae clinical isolates. However, in the last few
years, colistin-resistant A. baumannii and K. pneumoniae strains have emerged.
Therefore, combination therapies, between colistin and other old drugs, restoring
the activity of colistin are required. The main objective of this study was to
analyse the activity of niclosamide, an anthelmintic drug, in combination with colistin
against colistin-susceptible (Col-S) and colistin-resistant (Col-R) A. baumannii and
K. pneumoniae. The MIC were determined by microdilution assay and the time-kill
curves were performed. The zeta potential of Col-S and Col-R of A. baumannii and
K. pneumoniae in presence of niclosamide was assessed. Niclosamide in combination
with colistin showed improved activity against Col-S and Col-R A. baumannii and
K. pneumoniae. Time-killing curves showed synergic activity between niclosamide and
colistin against Col-S and Col-R A. baumannii and K. pneumoniae, especially when
niclosamide or colistin was added for second time at 4 h of the 24 h killing curve. Col-R
A. baumannii and K. pneumoniae in presence of niclosamide exhibited a greater negative
charge (−34.95 ± 0.35 mV and −38.85 ± 0.92 mV; P < 0.05) than Col-R A. baumannii
and K. pneumoniae in absence of niclosamide (−26.85 ± 3.65 mV and −35.27 ±
0.72 mV). These data suggest that niclosamide might be combined with colistin, being a
potential alternative for treatment of Col-R Gram-negative bacilli infections. | es |
dc.description.sponsorship | Instituto de Salud Carlos III | es |
dc.description.sponsorship | Proyectos de Investigacion en Salud PI16/01378 | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad CP15/01358 | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Research Foundation | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Repurposing drug | es |
dc.subject | Niclosamide | es |
dc.subject | Colistin | es |
dc.subject | Synergistic effect | es |
dc.subject | Acinetobacter baumannii | es |
dc.subject | Klebsiella pneumoniae | es |
dc.title | Synergistic Activity of Niclosamide in Combination With Colistin Against Colistin-Susceptible and Colistin-Resistant Acinetobacter baumannii and Klebsiella pneumoniae | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Medicina | es |
dc.relation.projectID | PI16/01378 | es |
dc.relation.projectID | CP15/01358 | es |
dc.identifier.doi | 10.3389/fcimb.2018.00348 | es |
dc.journaltitle | Frontiers in cellular and infection microbiology | es |
dc.publication.volumen | 8 | es |