Artículo
Harmful DNA:RNA hybrids are formed in cis and in a Rad51-independent manner
Autor/es | Lafuente Barquero, Juan
García Rubio, María Luisa San Martín Alonso, Marta Gómez González, Belén Aguilera López, Andrés |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2020 |
Fecha de depósito | 2020-09-25 |
Publicado en |
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Resumen | DNA:RNA hybrids constitute a well-known source of recombinogenic DNA damage. The current literature is in agreement with DNA:RNA hybrids being produced co-transcriptionally by the invasion of the nascent RNA molecule ... DNA:RNA hybrids constitute a well-known source of recombinogenic DNA damage. The current literature is in agreement with DNA:RNA hybrids being produced co-transcriptionally by the invasion of the nascent RNA molecule produced in cis with its DNA template. However, it has also been suggested that recombinogenic DNA:RNA hybrids could be facilitated by the invasion of RNA molecules produced in trans in a Rad51-mediated reaction. Here, we tested the possibility that such DNA:RNA hybrids constitute a source of recombinogenic DNA damage taking advantage of Rad51-independent single-strand annealing (SSA) assays in the yeast Saccharomyces cerevisiae. For this, we used new constructs designed to induce expression of mRNA transcripts in trans with respect to the SSA system. We show that unscheduled and recombinogenic DNA:RNA hybrids that trigger the SSA event are formed in cis during transcription and in a Rad51-independent manner. We found no evidence that such hybrids form in trans and in a Rad51-dependent manner. |
Identificador del proyecto | BFU2016-75058-P |
Cita | Lafuente Barquero, J., García Rubio, M.L., San Martín Alonso, M., Gómez González, B. y Aguilera López, A. (2020). Harmful DNA:RNA hybrids are formed in cis and in a Rad51-independent manner. eLife, 9, e56674-. |
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Harmful DNA RNA hybrids.pdf | 966.7Kb | [PDF] | Ver/ | |