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dc.creatorMartín Hernánz, Saraes
dc.creatorAparicio Martínez, Abelardoes
dc.creatorFernández Mazuecos, Marioes
dc.creatorRubio Pérez, Encarnaciónes
dc.creatorReyes Betancort, J. Alfredoes
dc.creatorSantos Guerra, Arnoldoes
dc.creatorOlangua Corral, Maríaes
dc.creatorGonzález Albaladejo, Rafaeles
dc.date.accessioned2019-12-02T11:31:07Z
dc.date.available2019-12-02T11:31:07Z
dc.date.issued2019
dc.identifier.citationMartín Hernánz, S., Aparicio Martínez, A., Fernández Mazuecos, M., Rubio Pérez, E., Reyes Betancort, J.A., Santos Guerra, A.,...,González Albaladejo, R. (2019). Maximize Resolution or Minimize Error? Using Genotyping-By-Sequencing to Investigate the Recent Diversification of Helianthemum (Cistaceae). Frontiers in Plant Science, 10 (| art.1416), 1-21.
dc.identifier.issn1664-462Xes
dc.identifier.urihttps://hdl.handle.net/11441/90660
dc.description.abstractA robust phylogenetic framework, in terms of extensive geographical and taxonomic sampling, well-resolved species relationships and high certainty of tree topologies and branch length estimations, is critical in the study of macroevolutionary patterns. Whereas Sanger sequencing-based methods usually recover insufficient phylogenetic signal, especially in recently diversified lineages, reduced-representation sequencing methods tend to provide well-supported phylogenetic relationships, but usually entail remarkable bioinformatic challenges due to the inherent trade-off between the number of SNPs and the magnitude of associated error rates. The genus Helianthemum (Cistaceae) is a species-rich and taxonomically complex Palearctic group of plants that diversified mainly since the Upper Miocene. It is a challenging case study since previous attempts using Sanger sequencing were unable to resolve the intrageneric phylogenetic relationships. Aiming to obtain a robust phylogenetic reconstruction based on genotyping-by-sequencing (GBS), we established a rigorous methodological workflow in which we i) explored how variable settings during dataset assembly have an impact on error rates and on the degree of resolution under concatenation and coalescent approaches, ii) assessed the effect of two extreme parameter configurations (minimizing error rates vs. maximizing phylogenetic resolution) on tree topology and branch lengths, and iii) evaluated the effects of these two configurations on estimates of divergence times and diversification rates. Our analyses produced highly supported topologically congruent phylogenetic trees for both configurations. However, minimizing error rates did produce more reliable branch lengths, critically affecting the accuracy of downstream analyses (i.e. divergence times and diversification rates). In addition to recommending a revision of intrageneric systematics, our results enabled us to identify three highly diversified lineages in Helianthemum in contrasting geographical areas and ecological conditions, which started radiating in the Upper Miocene.es
dc.description.sponsorshipEspaña, MINECO grants CGL2014- 52459-P and CGL2017-82465-Pes
dc.description.sponsorshipEspaña, Ministerio de Economía, Industria y Competitividad, reference IJCI-2015-23459es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.ispartofFrontiers in Plant Science, 10 (| art.1416), 1-21.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHelianthemumes
dc.subjectbranch lengthes
dc.subjectdiversificationes
dc.subjectevolutionary radiationes
dc.subjectgenotyping-by-sequencinges
dc.subjectphylogenetic resolutiones
dc.subjectphylogenomicses
dc.titleMaximize Resolution or Minimize Error? Using Genotyping-By-Sequencing to Investigate the Recent Diversification of Helianthemum (Cistaceae)es
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Vegetal y Ecologíaes
dc.relation.projectIDCGL2014- 52459-P and CGL2017-82465-Pes
dc.relation.projectIDIJCI-2015-23459es
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fpls.2019.01416es
dc.identifier.doi10.3389/fpls.2019.01416es
idus.format.extent21 p.es
dc.journaltitleFrontiers in Plant Sciencees
dc.publication.volumen10es
dc.publication.issue| art.1416es
dc.publication.initialPage1es
dc.publication.endPage21es

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional