Artículo
Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heatand Metallo-Resistant PGPR
Autor/es | Raklami, Anas
Oufdou, Khalid Tahiri, Abdel-Ilah Mateos Naranjo, Enrique Navarro de la Torre, Salvadora Rodríguez Llorente, Ignacio David Meddich, Abdelilah Redondo Gómez, Susana Pajuelo Domínguez, Eloísa |
Departamento | Universidad de Sevilla. Departamento de Microbiología y Parasitología Universidad de Sevilla. Departamento de Biología Vegetal y Ecología |
Fecha de publicación | 2019 |
Fecha de depósito | 2019-08-26 |
Publicado en |
|
Resumen | Soil contamination with heavy metals is a constraint for plant establishment and development
for which phytoremediation may be a solution, since rhizobacteria may alleviate plant stress under
these conditions. A greenhouse ... Soil contamination with heavy metals is a constraint for plant establishment and development for which phytoremediation may be a solution, since rhizobacteria may alleviate plant stress under these conditions. A greenhouse experiment was conducted to elucidate the effect of toxic metals on growth, the activities of ROS (reactive oxygen species)-scavenging enzymes, and gene expression of Medicago sativa grown under different metal and/or inoculation treatments. The results showed that, besides reducing biomass, heavy metals negatively affected physiological parameters such as chlorophyll fluorescence and gas exchange, while increasing ROS-scavenging enzyme activities. Inoculation of M. sativa with a bacterial consortium of heat- and metallo-resistant bacteria alleviated metal stress, as deduced from the improvement of growth, lower levels of antioxidant enzymes, and increased physiological parameters. The bacteria were able to effectively colonize and form biofilms onto the roots of plants cultivated in the presence of metals, as observed by scanning electron microscopy. Results also evidenced the important role of glutathione reductase (GR), phytochelatin synthase (PCS), and metal transporter NRAMP1 genes as pathways for metal stress management, whereas the gene coding for cytochrome P450 (CP450) seemed to be regulated by the presence of the bacteria. These outcomes showed that the interaction of metal-resistant rhizobacteria/legumes can be used as an instrument to remediate metal-contaminated soils, while cultivation of inoculated legumes on these soils is still safe for animal grazing, since inoculation with bacteria diminished the concentrations of heavy metals accumulated in the aboveground parts of the plants to below toxic levels |
Identificador del proyecto | PPR2 /2016/42
CGL2016-75550-R |
Cita | Raklami, A., Oufdou, K., Tahiri, A., Mateos Naranjo, E., Navarro de la Torre, S., Rodríguez Llorente, I.D.,...,Pajuelo Domínguez, E. (2019). Safe Cultivation of Medicago sativa in Metal-Polluted Soils from Semi-Arid Regions Assisted by Heatand Metallo-Resistant PGPR. Microorganism, 7 (7), 212. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
microorganisms-07-00212-v2.pdf | 4.479Mb | [PDF] | Ver/ | |