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A pyrF auxotrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interactions with soybean and other legumes

 

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Opened Access A pyrF auxotrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interactions with soybean and other legumes
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Author: Crespo Rivas, Juan Carlos
Margaret Oliver, Isabel María
Pérez Montaño, Francisco de Asís
López Baena, Francisco Javier
Vinardell González, José María
Ollero Márquez, Francisco Javier
Moreno Onorato, Francisco Javier
Ruiz Sainz, José Enrique
Buendía Clavería, Ana María
Department: Universidad de Sevilla. Departamento de Microbiología
Date: 2007
Document type: Article
Abstract: Transposon Tn5-Mob mutagenesis allowed the selection of a Sinorhizobium fredii HH103 mutant derivative (SVQ 292) that requires the presence of uracil to grow in minimal media. The mutated gene, pyrF, codes for an orotidine-5´- monophosphate decarboxylase (EC 4.1.1.23). Mutant SVQ 292 and its parental prototrophic mutant HH103 showed similar Nod-factor and lipopolysaccharide profiles. The symbiotic properties of mutant SVQ 292 were severely impaired with all legumes tested. Mutant SVQ 292 formed small ineffective nodules on Cajanus cajan and abnormal nodules (pseudonodules) unable to fix nitrogen on Glycine max (soybean), Macroptitlium atropurpureum, Indigofera tinctoria, and Desmodium canadense. It also did not induce any macroscopic response in Macrotyloma axillare roots. The symbiotic capacity of SVQ 292 with soybean was not enhanced by the addition of uracil to the plant nutritive solution
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URI: https://hdl.handle.net/11441/87928

DOI: 10.2436/20.1501.01.24

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