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Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3

 

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dc.creator Cabello Valverde, Miguel es
dc.creator Ge, Haobo es
dc.creator Aracil Fernández, Carmen es
dc.creator Moschou, Despina es
dc.creator Estrela, Pedro es
dc.creator Quero Reboul, José Manuel es
dc.creator Pascu, Sofia I. es
dc.creator Rocha, Paulo R. F. es
dc.date.accessioned 2019-04-16T08:45:26Z
dc.date.available 2019-04-16T08:45:26Z
dc.date.issued 2019-01
dc.identifier.citation Cabello Valverde, M., Ge, H., Aracil Fernández, C., Moschou, D., Estrela, P., Quero Reboul, J.M.,...,Rocha, P.R.F. (2019). Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3. Sensors, 19 (1)
dc.identifier.issn 1424-8220 es
dc.identifier.uri https://hdl.handle.net/11441/85691
dc.description.abstract Although prostate cancer is one of the most common cancers in the male population, its basic biological function at a cellular level remains to be fully understood. This lack of in depth understanding of its physiology significantly hinders the development of new, targeted and more effective treatment strategies. Whilst electrophysiological studies can provide in depth analysis, the possibility of recording electrical activity in large populations of non-neuronal cells remains a significant challenge, even harder to address in the picoAmpere-range, which is typical of cellular level electrical activities. In this paper, we present the measurement and characterization of electrical activity of populations of prostate cancer cells PC-3, demonstrating for the first time a meaningful electrical pattern. The low noise system used comprises a multi-electrode array (MEA) with circular gold electrodes on silicon oxide substrates. The extracellular capacitive currents present two standard patterns: an asynchronous sporadic pattern and a synchronous quasi-periodic biphasic spike pattern. An amplitude of ±150 pA, a width between 50–300 ms and an inter-spike interval around 0.5 Hz characterize the quasi-periodic spikes. Our experiments using treatment of cells with Gd3+, known as an inhibitor for the Ca2+ exchanges, suggest that the quasi-periodic signals originate from Ca2 channels. After adding the Gd3+ to a population of living PC-3 cells, their electrical activity considerably decreased; once the culture was washed, thus eliminating the Gd3+ containing medium and addition of fresh cellular growth medium, the PC-3 cells recovered their normal electrical activity. Cellular viability plots have been carried out, demonstrating that the PC-3 cells remain viable after the use of Gd3+, on the timescale of this experiment. Hence, this experimental work suggests that Ca2+ is significantly affecting the electrophysiological communication pattern among PC-3 cell populations. Our measuring platform opens up new avenues for real time and highly sensitive investigations of prostate cancer signalling pathways. es
dc.description.sponsorship Ministerio de Ciencia e Innovación TEC2014-54449-C3-2-R, BIOLOP es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher MDPI es
dc.relation.ispartof Sensors, 19 (1)
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Prostate cancer signalling es
dc.subject PC-3 cells es
dc.subject Electrical activity es
dc.subject Calcium channel inhibitor es
dc.title Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3 es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Ingeniería Electrónica es
dc.relation.projectID TEC2014-54449-C3-2-R, BIOLOP es
dc.relation.publisherversion https://doi.org/10.3390/s19010139 es
dc.identifier.doi 10.3390/s19010139 es
idus.format.extent 11 p. es
dc.journaltitle Sensors es
dc.publication.volumen 19 es
dc.publication.issue 1 es
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