dc.creator | Cabello Valverde, Miguel | es |
dc.creator | Ge, Haobo | es |
dc.creator | Aracil Fernández, Carmen | es |
dc.creator | Moschou, Despina | es |
dc.creator | Estrela, Pedro | es |
dc.creator | Quero Reboul, José Manuel | es |
dc.creator | Pascu, Sofia I. | es |
dc.creator | Rocha, Paulo R. F. | es |
dc.date.accessioned | 2019-04-16T08:45:26Z | |
dc.date.available | 2019-04-16T08:45:26Z | |
dc.date.issued | 2019-01 | |
dc.identifier.citation | Cabello Valverde, M., Ge, H., Aracil Fernández, C., Moschou, D., Estrela, P., Quero Reboul, J.M.,...,Rocha, P.R.F. (2019). Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3. Sensors, 19 (1) | |
dc.identifier.issn | 1424-8220 | es |
dc.identifier.uri | https://hdl.handle.net/11441/85691 | |
dc.description.abstract | Although prostate cancer is one of the most common cancers in the male population,
its basic biological function at a cellular level remains to be fully understood. This lack of in
depth understanding of its physiology significantly hinders the development of new, targeted
and more effective treatment strategies. Whilst electrophysiological studies can provide in depth
analysis, the possibility of recording electrical activity in large populations of non-neuronal cells
remains a significant challenge, even harder to address in the picoAmpere-range, which is typical
of cellular level electrical activities. In this paper, we present the measurement and characterization
of electrical activity of populations of prostate cancer cells PC-3, demonstrating for the first time a
meaningful electrical pattern. The low noise system used comprises a multi-electrode array (MEA)
with circular gold electrodes on silicon oxide substrates. The extracellular capacitive currents present
two standard patterns: an asynchronous sporadic pattern and a synchronous quasi-periodic biphasic
spike pattern. An amplitude of ±150 pA, a width between 50–300 ms and an inter-spike interval
around 0.5 Hz characterize the quasi-periodic spikes. Our experiments using treatment of cells with
Gd3+, known as an inhibitor for the Ca2+ exchanges, suggest that the quasi-periodic signals originate
from Ca2
channels. After adding the Gd3+ to a population of living PC-3 cells, their electrical activity
considerably decreased; once the culture was washed, thus eliminating the Gd3+ containing medium
and addition of fresh cellular growth medium, the PC-3 cells recovered their normal electrical activity.
Cellular viability plots have been carried out, demonstrating that the PC-3 cells remain viable after the
use of Gd3+, on the timescale of this experiment. Hence, this experimental work suggests that Ca2+ is
significantly affecting the electrophysiological communication pattern among PC-3 cell populations.
Our measuring platform opens up new avenues for real time and highly sensitive investigations of
prostate cancer signalling pathways. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación TEC2014-54449-C3-2-R, BIOLOP | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Sensors, 19 (1) | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Prostate cancer signalling | es |
dc.subject | PC-3 cells | es |
dc.subject | Electrical activity | es |
dc.subject | Calcium channel inhibitor | es |
dc.title | Extracellular Electrophysiology in the Prostate Cancer Cell Model PC-3 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Ingeniería Electrónica | es |
dc.relation.projectID | TEC2014-54449-C3-2-R, BIOLOP | es |
dc.relation.publisherversion | https://doi.org/10.3390/s19010139 | es |
dc.identifier.doi | 10.3390/s19010139 | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Sensors | es |
dc.publication.volumen | 19 | es |
dc.publication.issue | 1 | es |