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N-terminal chimaeras with signal sequences enhance the functional expression and alter the subcellular localization of heterologous membrane-bound inorganic pyrophosphatases in yeast

 

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Opened Access N-terminal chimaeras with signal sequences enhance the functional expression and alter the subcellular localization of heterologous membrane-bound inorganic pyrophosphatases in yeast
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Author: Drake García, Rocío
Serrano Delgado, Aurelio
Pérez Castiñeira, José Román
Department: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Date: 2010
Published in: Biochemical Journal, 426 (2), 147-157.
Document type: Article
Abstract: Expression of heterologous multispanning membrane proteins in Saccharomyces cerevisiae is a difficult task. Quite often, the use of multicopy plasmids where the foreign gene is under the control of a strong promoter does not guarantee efficient prod...
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Cite: Drake García, R., Serrano Delgado, A. y Pérez Castiñeira, J.R. (2010). N-terminal chimaeras with signal sequences enhance the functional expression and alter the subcellular localization of heterologous membrane-bound inorganic pyrophosphatases in yeast. Biochemical Journal, 426 (2), 147-157.
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URI: https://hdl.handle.net/11441/85283

DOI: 10.1042/BJ20091491

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