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Osmotic stress activates nif and fix genes and induces the Rhizobium tropici CIAT 899 Nod factor production via NodD2 by up-regulation of the nodA2 operon and the nodA3 gene

 

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dc.creator Cerro Sánchez, Pablo del es
dc.creator Megías Guijo, Manuel es
dc.creator López Baena, Francisco Javier es
dc.creator Gil Serrano, Antonio Miguel es
dc.creator Pérez Montaño, Francisco de Asís es
dc.creator Ollero Márquez, Francisco Javier es
dc.date.accessioned 2019-04-04T08:14:16Z
dc.date.available 2019-04-04T08:14:16Z
dc.date.issued 2019
dc.identifier.citation Cerro Sánchez, P.d., Megías Guijo, M., López Baena, F.J., Gil Serrano, A.M., Pérez Montaño, F.d.A. y Ollero Márquez, F.J. (2019). Osmotic stress activates nif and fix genes and induces the Rhizobium tropici CIAT 899 Nod factor production via NodD2 by up-regulation of the nodA2 operon and the nodA3 gene. PLoS One, 14 (3), e0213298-1-e0213298-23.
dc.identifier.issn 1932-6203 es
dc.identifier.uri https://hdl.handle.net/11441/85193
dc.description.abstract The symbiosis between rhizobia and legumes is characterized by a complex molecular dialogue in which the bacterial NodD protein plays a major role due to its capacity to activate the expression of the nodulation genes in the presence of appropiate flavonoids. These genes are involved in the synthesis of molecules, the nodulation factors (NF), responsible for launching the nodulation process. Rhizobium tropici CIAT 899, a rhizobial strain that nodulates Phaseolus vulgaris, is characterized by its tolerance to multiple environmental stresses such as high temperatures, acidity or elevated osmolarity. This strain produces nodulation factors under saline stress and the same set of CIAT 899 nodulation genes activated by inducing flavonoids are also up-regulated in a process controlled by the NodD2 protein. In this paper, we have studied the effect of osmotic stress (high mannitol concentrations) on the R. tropici CIAT 899 transcriptomic response. In the same manner as with saline stress, the osmotic stress mediated NF production and export was controlled directly by NodD2. In contrast to previous reports, the nodA2FE operon and the nodA3 and nodD1 genes were up-regulated with mannitol, which correlated with an increase in the production of biologically active NF. Interestingly, in these conditions, this regulatory protein controlled not only the expression of nodulation genes but also the expression of other genes involved in protein folding and synthesis, motility, synthesis of polysaccharides and, surprinsingly, nitrogen fixation. Moreover, the non-metabolizable sugar dulcitol was also able to induce the NF production and the activation of nod genes in CIAT 899. es
dc.description.sponsorship España, MINECO AGL2016-77163-R es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Public Library of Science es
dc.relation.ispartof PLoS One, 14 (3), 1-23.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Osmotic stress activates nif and fix genes and induces the Rhizobium tropici CIAT 899 Nod factor production via NodD2 by up-regulation of the nodA2 operon and the nodA3 gene es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Química Orgánica es
dc.relation.projectID AGL2016-77163-R es
dc.relation.publisherversion http://dx.doi.org/10.1371/journal.pone.0213298 es
dc.identifier.doi 10.1371/journal.pone.0213298 es
idus.format.extent 23 p. es
dc.journaltitle PLoS One es
dc.publication.volumen 14 es
dc.publication.issue 3 es
dc.publication.initialPage e0213298-1 es
dc.publication.endPage e0213298-23 es
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