Mostrar el registro sencillo del ítem

Artículo

dc.creatorBahaji, Abdellatifes
dc.creatorBaroja Fernández, Edurnees
dc.creatorRicarte Bermejo, Adrianaes
dc.creatorSánchez López, Ángela Maríaes
dc.creatorMuñoz Pérez, Francisco Josées
dc.creatorRomero Rodríguez, José Maríaes
dc.creatorRuiz Pérez, María Teresaes
dc.creatorBaslam, Marouanees
dc.creatorAlmagro, Goizederes
dc.creatorSesma, María Teresaes
dc.creatorPozueta Romero, Javieres
dc.date.accessioned2019-04-01T10:40:44Z
dc.date.available2019-04-01T10:40:44Z
dc.date.issued2015
dc.identifier.citationBahaji, A., Baroja Fernández, E., Ricarte Bermejo, A., Sánchez López, Á.M., Muñoz Pérez, F.J., Romero Rodríguez, J.M.,...,Pozueta Romero, J. (2015). Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis. Plant Science, 238, 135-147.
dc.identifier.issn0168-9452es
dc.identifier.urihttps://hdl.handle.net/11441/84990
dc.description.abstractWe characterized multiple knock-out mutants of the four Arabidopsis sucrose phosphate synthase (SPSA1, SPSA2, SPSB and SPSC) isoforms. Despite their reduced SPS activity, spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc mutants displayed wild type (WT) vegetative and reproductive morphology, and showed WT photosynthetic capacity and respiration. In contrast, growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants was reduced compared with WT plants. Furthermore, these plants displayed a high dark respiration phenotype. spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinated and produced aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulated WT levels of nonstructural carbohydrates. spsa1/spsc leaves possessed high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulated high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions. Results presented in this work show that SPS is essential for plant viability, reveal redundant functions of the four SPS isoforms in processes that are important for plant growth and nonstructural carbohydrate metabolism, and strongly indicate that accelerated starch turnover and enhanced respiration can alleviate the blockage of sucrose biosynthesis in spsa1/spsc leaves.es
dc.description.sponsorshipComisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional BIO2010-18239 BIO2013-49125-C2-1-P BIO2008-02292 BIO2011-28847-C02-02es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofPlant Science, 238, 135-147.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCarbohydrate metabolismes
dc.subjectDevelopmentes
dc.subjectFunctional interactiones
dc.subjectGenetic redundancyes
dc.subjectGrowthes
dc.subjectSucrosees
dc.titleCharacterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesises
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBIO2010-18239es
dc.relation.projectIDBIO2013-49125-C2-1-Pes
dc.relation.projectIDBIO2008-02292es
dc.relation.projectIDBIO2011-28847-C02-02es
dc.relation.publisherversionhttps://doi.org/10.1016/j.plantsci.2015.06.009es
dc.identifier.doi10.1016/j.plantsci.2015.06.009es
idus.format.extent13 p.es
dc.journaltitlePlant Sciencees
dc.publication.volumen238es
dc.publication.initialPage135es
dc.publication.endPage147es

FicherosTamañoFormatoVerDescripción
Characterization of multiple SPS ...1.051MbIcon   [PDF] Ver/Abrir  

Este registro aparece en las siguientes colecciones

Mostrar el registro sencillo del ítem

Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Excepto si se señala otra cosa, la licencia del ítem se describe como: Attribution-NonCommercial-NoDerivatives 4.0 Internacional