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Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis

 

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dc.creator Bahaji, Abdellatif es
dc.creator Baroja Fernández, Edurne es
dc.creator Ricarte Bermejo, Adriana es
dc.creator Sánchez López, Ángela María es
dc.creator Muñoz Pérez, Francisco José es
dc.creator Romero Rodríguez, José María es
dc.creator Ruiz Pérez, María Teresa es
dc.creator Baslam, Marouane es
dc.creator Almagro, Goizeder es
dc.creator Sesma, María Teresa es
dc.creator Pozueta Romero, Javier es
dc.date.accessioned 2019-04-01T10:40:44Z
dc.date.available 2019-04-01T10:40:44Z
dc.date.issued 2015
dc.identifier.citation Bahaji, A., Baroja Fernández, E., Ricarte Bermejo, A., Sánchez López, Á.M., Muñoz Pérez, F.J., Romero Rodríguez, J.M.,...,Pozueta Romero, J. (2015). Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis. Plant Science, 238, 135-147.
dc.identifier.issn 0168-9452 es
dc.identifier.uri https://hdl.handle.net/11441/84990
dc.description.abstract We characterized multiple knock-out mutants of the four Arabidopsis sucrose phosphate synthase (SPSA1, SPSA2, SPSB and SPSC) isoforms. Despite their reduced SPS activity, spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc mutants displayed wild type (WT) vegetative and reproductive morphology, and showed WT photosynthetic capacity and respiration. In contrast, growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants was reduced compared with WT plants. Furthermore, these plants displayed a high dark respiration phenotype. spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinated and produced aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulated WT levels of nonstructural carbohydrates. spsa1/spsc leaves possessed high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulated high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions. Results presented in this work show that SPS is essential for plant viability, reveal redundant functions of the four SPS isoforms in processes that are important for plant growth and nonstructural carbohydrate metabolism, and strongly indicate that accelerated starch turnover and enhanced respiration can alleviate the blockage of sucrose biosynthesis in spsa1/spsc leaves. es
dc.description.sponsorship Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional BIO2010-18239 BIO2013-49125-C2-1-P BIO2008-02292 BIO2011-28847-C02-02 es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Elsevier es
dc.relation.ispartof Plant Science, 238, 135-147.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Carbohydrate metabolism es
dc.subject Development es
dc.subject Functional interaction es
dc.subject Genetic redundancy es
dc.subject Growth es
dc.subject Sucrose es
dc.title Characterization of multiple SPS knockout mutants reveals redundant functions of the four Arabidopsis sucrose phosphate synthase isoforms in plant viability, and strongly indicates that enhanced respiration and accelerated starch turnover can alleviate the blockage of sucrose biosynthesis es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/acceptedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular es
dc.relation.projectID BIO2010-18239 es
dc.relation.projectID BIO2013-49125-C2-1-P es
dc.relation.projectID BIO2008-02292 es
dc.relation.projectID BIO2011-28847-C02-02 es
dc.relation.publisherversion https://doi.org/10.1016/j.plantsci.2015.06.009 es
dc.identifier.doi 10.1016/j.plantsci.2015.06.009 es
idus.format.extent 13 p. es
dc.journaltitle Plant Science es
dc.publication.volumen 238 es
dc.publication.initialPage 135 es
dc.publication.endPage 147 es
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