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dc.creatorYruela Guerrero, Inmaculadaes
dc.creatorGarcía Rubio, Inéses
dc.creatorRoncel Gil, Mercedeses
dc.creatorMartínez, Jesús I.es
dc.creatorRamiro Pascual, María Victoriaes
dc.creatorOrtega Rodríguez, José Maríaes
dc.creatorAlonso, Pablo J.es
dc.creatorPicorel Castaño, Rafaeles
dc.date.accessioned2019-03-29T12:09:11Z
dc.date.available2019-03-29T12:09:11Z
dc.date.issued2003
dc.identifier.citationYruela Guerrero, I., García Rubio, I., Roncel Gil, M., Martínez, J.I., Ramiro Pascual, M.V., Ortega Rodríguez, J.M.,...,Picorel Castaño, R. (2003). Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre. Photochemistry and Photobiology, 2 (4), 437-442.
dc.identifier.issn0031-8655 (impreso)es
dc.identifier.issn1751-1097 (electrónico)es
dc.identifier.urihttps://hdl.handle.net/11441/84937
dc.description.abstractDetergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1-D2-Cyt b559 complex preparations. Various n-dodecyl-β-D-maltoside concentrations from 0 to 0.2% (w/v) were used to stabilise the D1-D2-Cyt b559 complexes. Low spin heme EPR spectra were obtained but gz feature positions changed depending on detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (E ‘m = +123-150 mV) in all the D1-D2-Cyt b559complex preparations indicating that detergent does not affect this property of the protein in those conditions. Similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein.es
dc.description.sponsorshipDirección General de Investigación Científica y Técnica PB98-1632 PB97-1135es
dc.description.sponsorshipDiputación General de Aragón P17/98 P111/2001es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherWileyes
dc.relation.ispartofPhotochemistry and Photobiology, 2 (4), 437-442.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCytochrome b559es
dc.subjectDetergentes
dc.subjectElectron paramagnetic resonancees
dc.subjectPhotosystem IIes
dc.subjectReaction centrees
dc.subjectRedox potentiales
dc.titleDetergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centrees
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDPB98-1632es
dc.relation.projectIDPB97-1135es
dc.relation.projectIDP17/98es
dc.relation.projectIDP111/2001es
dc.relation.publisherversionhttp://dx.doi.org/10.1039/b300187ces
dc.identifier.doi10.1039/b300187ces
idus.format.extent6 p.es
dc.journaltitlePhotochemistry and Photobiologyes
dc.publication.volumen2es
dc.publication.issue4es
dc.publication.initialPage437es
dc.publication.endPage442es

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional